Strategies needed to isolate mutant cell lines and to use the mutants to clone and map the genes are presented in detail. This is the first book in which such methodology and molecular biology are combined.
The preferred cell lines for genetic analysis are presented in the first section, followed by special techniques for isolating a wide range of mutants. The remainder of the book is devoted to genetic mapping and cloning of mutant genes. The final section presents special techniques in gene regulation.
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"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced."
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"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work."
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