DNA Microarrays, Part B: Databases and Statistics (Volume 411) (Methods in Enzymology, Volume 411) - Hardcover

Book 62 of 272: ISSN
 
9780121828165: DNA Microarrays, Part B: Databases and Statistics (Volume 411) (Methods in Enzymology, Volume 411)
Hardcover
ISBN 10: 0121828166 ISBN 13: 9780121828165
Publisher: Academic Press, 2006

Synopsis

Modern DNA microarray technologies have evolved over the past 25 years to the point where it is now possible to take many million measurements from a single experiment. These two volumes, Parts A & B in the Methods in Enzymology series provide methods that will shepard any molecular biologist through the process of planning, performing, and publishing microarray results.

Part A starts with an overview of a number of microarray platforms, both commercial and academically produced and includes wet bench protocols for performing traditional expression analysis and derivative techniques such as detection of transcription factor occupancy and chromatin status. Wet-bench protocols and troubleshooting techniques continue into Part B. These techniques are well rooted in traditional molecular biology and while they require traditional care, a researcher that can reproducibly generate beautiful Northern or Southern blots should have no difficulty generating beautiful array hybridizations.

Data management is a more recent problem for most biologists. The bulk of Part B provides a range of techniques for data handling. This includes critical issues, from normalization within and between arrays, to uploading your results to the public repositories for array data, and how to integrate data from multiple sources. There are chapters in Part B for both the debutant and the expert bioinformatician.

  • Provides an overview of platforms
  • Includes experimental design and wet bench protocols
  • Presents statistical and data analysis methods, array databases, data visualization and meta analysis

"synopsis" may belong to another edition of this title.

Book Description

A comprehensive collection of methods and protocols for working with DNA microarray technologies.

"About this title" may belong to another edition of this title.

Publisher
Academic Press
Publication date
2006
Language
English
ISBN 10 
0121828166
ISBN 13 
9780121828165
Binding
Hardcover
Edition number
1
Number of pages
512
Editor
Kimmel Alan R., Oliver Brian