Cell Culture, Volume 2: Volume 2: Cell Culture (Methods in Neurosciences) (Vol 2) - Hardcover

Hardcover

ISBN 10: 0121852539 ISBN 13: 9780121852535

Publisher: Academic Press, 1990

Synopsis

The ability to assess the response of neural cells is an important goal of research in the neurosciences. The means to measure responses in a chemically defined medium is a positive feature of the use of cell culture and is the major reason its use is so important in this discipline.
Techniques for the purification of cells for culture, for large-scale isolation, for assessing differentiated function, and for tracing lineage are included. Many of the methods can easily be adapted to other systems.

Key Features
* Convenient benchtop format
* Methods presented for easy adaptation to new systems
* Comprehensive protocols included for
* Preparation and maintenance of primary and continuous cultures
* Cell purification
* Bulk isolation
* Studying pulsatility, growth on immobile carriers, rotation-mediated aggregation, microcultures, Maximow assembly cultures
* Assessment of differentiated and functional aspects: endocrine modulation, morphological variants, receptors, intracellular trafficking
* Clonal analysis and lineage analysis

About this title

About the Author

Edited by Michael P. Conn

Edition details

• Publisher: Academic Press
• Publication date: 1990
• Language: English
• ISBN 10: 0121852539
• ISBN 13: 9780121852535
• Binding: Hardcover
• Number of pages: 440
• Editor: Conn P. Michael