The Zebrafish: Biology, Volume 59 (Methods in Cell Biology)

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9780122121708: The Zebrafish: Biology, Volume 59 (Methods in Cell Biology)

This is the first in a two-volume, comprehensive treatment of the methodologies used in researching the zebrafish, an emerging vertebrate model system. The text includes discussions on development, genetic methodologies, and model applications.

Key Features
* Details state-of-the-art zebrafish protocols in a single-source reference
* Presents methods and reagents in user-friendly format
* Delineates critical steps and pitfalls of he procedures
* Illustrates techniques with full-color plates
Summarizes many new and interesting developmental mutants
* Includes appendices with strain information and a compendium of zebrafish World Wide Web sites
* Relevant to clinicians interested in vertebrate models of human congenital diseases

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About the Author:

Professor of Biochemistry and Marine Biology at Northeastern University, promoted 1996. Joined Northeastern faculty in 1987. Previously a faculty member in Dept. of Biochemistry at the University of Mississippi Medical Center, 1983-1987.Principal Investigator in the U.S. Antarctic Program since 1984. Twelve field seasons "on the ice" since 1981. Research conducted at Palmer Station, Antarctica, and McMurdo Station, Antarctica.Research areas: Biochemical, cellular, and physiological adaptation to low and high temperatures. Structure and function of cytoplasmic microtubules and microtubule-dependent motors from cold-adapted Antarctic fishes. Regulation of tubulin and globin gene expression in zebrafish and Antarctic fishes. Role of microtubules in morphogenesis of the zebrafish embryo. Developmental hemapoiesis in zebrafish and Antarctic fishes. UV-induced DNA damage and repair in Antarctic marine organisms.

Review:

"The tropical freshwater fish Daniorerio has become a model system for the genetic analysis of vertebrate development. These two volumes offer researchers comprehensive coverage of the laboratory protocols now available for studies of developmental or cell biology. The first volume focuses on gene expression and function, early embryonic development, and organogenesis. The second covers methods for identifying and mapping mutations, cloning the causative genes, and using zebrafish databases."
--SCIENCE (April 16,1999)

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