RNA Methodologies: A Laboratory Guide for Isolation and Characterization - Softcover

Unknown, Author

Softcover

ISBN 10: 0122497007 ISBN 13: 9780122497001

Publisher: Academic Press, 1993

Synopsis

This book is a collection of tried and tested laboratory protocols for the isolation and characterization of mammalian RNA. It studies cellular regulation using RNA as a parameter of gene express, offers RNA isolation strategies, and explains proper handling, storage, and manipulation of RNA.


* Studies cellular regulation using RNA as a parameter of Gene Expression
* Offers RNA isolation strategies
* Explains proper handling, storage, and manipulation of RNA

About this title

Book Description

The expanded third edition of this highly successful laboratory manual presents new technologies in RNA interference

From the Back Cover

The isolation of biologically competent and chemically stable RNA is central to molecular biology. There remains an academic imperative to unify the many complex facets of the characterization of cellular biochemistry, with particular regard to the use of RNA as a parameter of gene expression. This laboratory guide is a collection of tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA. The more noteworthy additions to this edition include a variety of RNA-based PCR techniques, such as differential display PCR and transcript quantification by competitive (mimic) PCR.
The focus of the book is on how these methodologies can be used in the study of transcriptional and posttranscriptional regulation of eukaryotic genes. The liberal incorporation of flow charts, tables, and graphs facilitates learning and assists in the planning phases of projects. Investigators are limited only by their own ingenuity.

Edition details

• Publisher: Academic Press
• Publication date: 1993
• Language: English
• ISBN 10: 0122497007
• ISBN 13: 9780122497001
• Binding: Plastic Comb
• Number of pages: 317