Functional Organization of the Nucleus, Vol. 35: A Laboratory Guide (Methods in Cell Biology) - Softcover
Synopsis
The complex problem of the relationship between nuclear structure and function demands a multidisciplinary, multifaceted approach. This laboratory guide is designed for researchers, from graduate students to professionals, who need detailed protocols and general discussions on a broad range of techniques. The volume presents a selection of different methodological approaches for the analysis of nuclear structure and function--from cytological to molecular to genetic. These include visualization of nucleic acid sequences using hybridization probes, visualization of proteins using immunological probes, isolation of chromatin fractions, mapping in vitro protein*b1DNA interactions, reconstitution of functional templates and nuclear substructures, and genetic approaches to identifying and characterizing chromosomal components.
Features two full-color plates illustrating in situ hybridization methods
Presents, for the first time in one place, varied experimental approaches for studying nuclear structure and function including
Analysis of macromolecular interactions
Reconstitution of functional complexes
Visualization of nucleic acids and proteins
Genetic methods
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Functional Organization of the Nucleus, Vol. 35 A Laboratory Guide
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Academic Press, 1992
ISBN 10: 0123219205
ISBN 13: 9780123219206
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Methods in Cell Biology: Functional Organization of the Nucleus - A Laboratory Guide
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