Colloidal Gold: Principles, Methods, and Applications, Vol. 2

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9780123339287: Colloidal Gold: Principles, Methods, and Applications, Vol. 2

Since its introduction in 1971, the development and application of colloidal gold as a marker in electron microscopy has been phenomenal. Colloidal gold has become the method of choice in immunocytochemistry and many areas of cell biology. This universal method is applicable to most microscopical systems including optical microscopy; scanning, transmission, and high voltage electron microscopy; photoelectron, photon, fluorescent darkfield, and epipolarization microscopy. Colloidal gold allows high and low resolution studies, enzyme and nucleic acid labeling, study of dynamic cellular processes, and virus detection. This book is among the first available to cover the principles and methodology of colloidal gold in microscopy.

Key Features
* Methods are described step by step, to enable researchers to learn these complex procedures solely by reference to these books
* Problems and limitations of techniques are discussed
* Guides users to avoid problems and choose the correct procedures for specific applications
* Contributors are eminent authorities in their fields

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From the Back Cover:

Since its introduction in 1971, the development and application of colloidal gold as a marker in electron microscopy has been phenomenal. This state-of-the art, multi-volume treatise provides researchers, technicians, teachers, and students with the most comprehensive coverage of the principles and methodology of colloidal gold microscopy available today.
Topics covered in Volume 2 include:
Methodology for the study of antigenicity in neurology, virology, exocytosis, cytoskeleton, and plant tissues.
* The use of colloidal gold in conjunction with negative staining, nucleic acid hybridization, and darkfield and epipolarization microscopy
* The detection of proteins with colloidal gold
Molecular interactions between gold and proteins
* The roles of tissue processing in colloidal gold methods
* Low temperature methods
Topics covered in Volume 1 include:
* Principles, methods, and applications of colloidal gold methodology in cytochemistry and immunochemistry
* Methods for preparing colloidal gold particles of different sizes
* Protein A-gold, protein G-gold, and lectin-gold techniques
* The use of resins and thin cryosections
* Multiple labeling

About the Author:

Dr. Hayat has published extensively in the fields of microscopy, cytology, immunohistochemistry, immunocytochemistry, and antigen retrieval methods. He is Distinguished Professor, Department of Biological Sciences, Kean University, Union, New Jersey, USA.

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M. A. Hayat (Editor)
Published by Academic Press (1989)
ISBN 10: 0123339286 ISBN 13: 9780123339287
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M. A. Hayat
Published by Academic Press (1989)
ISBN 10: 0123339286 ISBN 13: 9780123339287
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