Colloidal Gold: Principles, Methods, and Applications, Vol. 3

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9780123339294: Colloidal Gold: Principles, Methods, and Applications, Vol. 3

Since its introduction in 1971, the development and application of colloidal gold as a marker in electron microscopy has been phenomenal. This state-of-the art, multi-volume treatise provides researchers, technicians, teachers, and students with the most comprehensive coverage of the principles and methodology of colloidal gold microscopy available today.
Colloidal gold allows high and low resolution studies, enzyme and nucleic acid labeling, study of dynamic cellular processes, and virus detection.
This third volume completes Hayat's coverage of the principles and methodology of colloidal gold in microscopy. The three-volume set should become the standard reference in the field.

Key Features
* Among the first books to cover principles and methodology of colloidal gold in microscopy
* Describes methods step by step to enable researchers to learn these complex procedures solely by reference to the book
* Discusses problems and limitations of techniques
* Guides users to avoid problems and choose the correct procedures for specific applications
* Contributors are eminent authorities in their fields
Topics covered in Volume 3 include:
* Production of monoclonal and polyclonal antibodies
* Lowicryl, polar, and apolar resins
* Preserving and localizing antigenicity; sectioning; high-resolution morphological localization
* Diaminobenzidine-colloidal gold; microinjection of colloidal gold; double labeling with backscattered electrons; microwave irradiation
* Applications to biomedical studies
* Colloidal gold in conjunction with video-enhanced microscopy

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From the Back Cover:

Since its introduction in 1971, the development and application of colloidal gold as a marker in electron microscopy has been phenomenal. This state-of-the-art, multivolume treatise provides researchers, technicians, teachers, and students with the most comprehensive coverage of the principles and methodology of colloidal gold microscopy available today.
Topics covered in Volume 1 include:
* Principles, methods, and applications of colloidal gold methodology in cytochemistry and immunochemistry
* Methods for preparing colloidal gold particles of different sizes
* Protein A-gold, protein G-gold, and lectin-gold techniques
* The use of resins and thin cryosections
* Multiple labeling
Topics covered in Volume 2 include:
Methodology for the study of antigenicity in neurology, virology, exocytosis, cytoskeleton, and plant tissues.
* The use of colloidal gold in conjunction with negative staining, nucleic acid hybridization, and darkfield and epipolarization microscopy
* The detection of proteins with colloidal gold
Molecular interactions between gold and proteins
* The roles of tissue processing in colloidal gold methods
* Low temperature methods

About the Author:

Dr. Hayat has published extensively in the fields of microscopy, cytology, immunohistochemistry, immunocytochemistry, and antigen retrieval methods. He is Distinguished Professor, Department of Biological Sciences, Kean University, Union, New Jersey, USA.

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M. A. Hayat
Published by Academic Press Inc (1991)
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