PCR Applications: Protocols for Functional Genomics - Softcover

Softcover

ISBN 10: 0123721865 ISBN 13: 9780123721860

Publisher: Academic Press, 1999

Synopsis

PCR is the most powerful technique currently used in molecular biology. It enables the scientist to quickly replicate DNA and RNA on the benchtop. From its discovery in the early 80's, PCR has blossomed into a method that enables everything from ready mutation of DNA/RNA to speedy analysis of tens of thousands of nucleotide sequences daily.PCR Applications examines the latest developments in this field. It is the third book in the series, building on the previous publications PCR Protocols and PCR Strategies. The manual discusses techniques that focus on gene discovery, genomics, and DNA array technology, which are contributing factors to the now-occurring bioinformatics boom.

• Focuses on gene discovery, genomics, and DNA array technology
• Covers quantitative PCR techniques, including the use of standards and kinetic analysis
• Includes statistical refinement of primer design parameters
• Ilustrates techniques used in microscopic tissue samples, such as single cell PCR, whole cell PCR, laser capture microdissection, and in situ PCR

Entries provide information on:

• Nomenclature
• Expression
• Sequence analysis
• Structure and function
• Electrophysiology
• Parmacology
• Information retrieval

About this title

About the Author

Michael A. Innis, Chiron Corporation, Emeryville, California, U.S.A.
David H. Gelfand, Roche Molecular Systems, Alameda, California, U.S.A.
John J. Sninsky, Roche Molecular Systems, Alameda, California, U.S.A.

From the Back Cover

The polymerase chain reaction is the most powerful technique currently used in molecular biology laboratories. From its discovery in the early 80s, PCR has blossomed into a method that enables everything from ready mutation of DNA to speedy analysis of tens of thousands of nucleotide sequences daily.
PCR Applications: Protocols for Functional Genomics examines the latest PCR implementations. It complements the other books in the series, PCR Protocols (1990) and PCR Strategies (1995), and techniques discussed are relevant to the now-occurring bioinformatics and proteomics boom.
@introbul:Key Features:
@bul:* focuses on gene discovery, genomics, and DNA array technology
* covers quantitative PCR techniques, including the use of standards and kinetic analysis
* includes statistical refinement of primer design parameters
* illustrates techniques used in microscopic tissue samples, such as single cell PCR, whole cell PCR, laser capture microdissection, and in situ PCR

Edition details

• Publisher: Academic Press
• Publication date: 1999
• Language: English
• ISBN 10: 0123721865
• ISBN 13: 9780123721860
• Binding: Plastic Comb
• Edition number: 1
• Number of pages: 566
• Editor: Innis Michael A., Gelfand David H., Sninsky John J.