A Practical Guide To The Study Of Calcium In Living Cells: Volume 40 - Softcover

9780124156814: A Practical Guide To The Study Of Calcium In Living Cells: Volume 40

Softcover

ISBN 10: 0124156819 ISBN 13: 9780124156814

Publisher: Academic Press, 2013

A Practical Guide to the Study of Calcium in Living Cells describes popular techniques along with helpful do's and don't's and computer programs. The volume enables investigators to evaluate confocal images, use the latest dyes, and design Calcium buffers appropriate to their research needs. This book is designed for laboratory use by graduate students, technicians, and researchers in many disciplines, ranging from molecular to cellular levels of investigation.

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From the Back Cover

This volume in the acclaimed seriesMethods in Cell Biology is designed for students and researchers in biology and biomedicine.A Practical Guide to the Study of Calcium in Living Cells describes highly sensitive techniques for measuring the calcium vital to many important cellular functions. This manual enables investigators to evaluate confocal images, use the latest dyes, and design Ca2+buffers appropriate to their research needs.
Highlights
* Describes techniques to
* Detect Ca2+with sensitive microelectrodes, fluorescent dyes, and luminescent proteins
* Perturb intracellular Ca2+
* Prepare Ca2+buffers with an easy-to-use computer program

* Contains color plates that illustrate confocal ratio-imaging and the use of aequorin
* Covers methods in detail and describes the problems and pitfalls of each technique

Review

"This multi-authored book... provides theoretical and applied aspects of different established techniques for the measurement of [Ca2+]i at submicromolar concentration. Each chapter is organized, keeping in view the needs of a person working in the laboratory, by giving the detailed methodology required for the implementation of that particular technique, and all the chapters are well referenced... The book provides a pragmatic approach for the estimation of cytosolic [Ca2+]i and also provides a means for studying the spatial and temporal resolution of [Ca2+]i. The book achieves the stated goal in the preface, 'this material will be very useful to the investigator who wants to apply any of these methods to his or her research.' It is a very well-written monograph and includes useful illustrations and helpful, practical points." --RAMESH BHALLA, University of Iowa, Iowa City

"The book achieves the stated goal in the preface, this material will be very useful to the investigator who wants to apply any of these methods to his or her research. It is a very well-written monograph and includes useful illustrations and helpful, practical points." --MOLECULAR & CELLULAR NEUROSCIENCES

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