A Practical Guide to Protein and Peptide Purification for Microsequencing, Second Edition

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9780124802827: A Practical Guide to Protein and Peptide Purification for Microsequencing, Second Edition

Why a Second Edition?
The Second Edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" rather than the more specific question asked in the first edition, "How can I obtain the N-terminal sequence?" Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry.

Key Features
Highlights of the Second Edition:
* Mass spectrophotometric characterization of proteins and peptides
* N-terminal sequencing of proteins with blocked N termini
* Internal amino acid sequence analysis after protease digestion in-gel and on-blot
* Improved microscale peptide purification methods
* Computer analysis of protein sequences
* New protocols tested and refined through everyday use in authors' laboratories
* Updated reference chapter covering all aspects of protein microsequencing

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Book Description Elsevier Science Publishing Co Inc, United States, 1993. Paperback. Book Condition: New. 2nd Revised edition. 226 x 152 mm. Language: English . Brand New Book ***** Print on Demand *****. Why a Second Edition? The Second Edition provides practical answers to the general question, How can I obtain useful sequence information from my protein or peptide? rather than the more specific question asked in the first edition, How can I obtain the N-terminal sequence? Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry. * Mass spectrophotometric characterization of proteins and peptides* N-terminal sequencing of proteins with blocked N termini* Internal amino acid sequence analysis after protease digestion in-gel and on-blot* Improved microscale peptide purification methods* Computer analysis of protein sequences* New protocols tested and refined through everyday use in authors laboratories* Updated reference chapter covering all aspects of protein microsequencing. Bookseller Inventory # AAV9780124802827

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