A Practical Guide to Protein and Peptide Purification for Microsequencing, Second Edition

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9780124802827: A Practical Guide to Protein and Peptide Purification for Microsequencing, Second Edition

Why a Second Edition?
The Second Edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" rather than the more specific question asked in the first edition, "How can I obtain the N-terminal sequence?" Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry.

Key Features
Highlights of the Second Edition:
* Mass spectrophotometric characterization of proteins and peptides
* N-terminal sequencing of proteins with blocked N termini
* Internal amino acid sequence analysis after protease digestion in-gel and on-blot
* Improved microscale peptide purification methods
* Computer analysis of protein sequences
* New protocols tested and refined through everyday use in authors' laboratories
* Updated reference chapter covering all aspects of protein microsequencing

"synopsis" may belong to another edition of this title.

Product Description:

Why a Second Edition?
The Second Edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" rather than the more specific question asked in the first edition, "How can I obtain the N-terminal sequence?" Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry.

  • Mass spectrophotometric characterization of proteins and peptides
  • N-terminal sequencing of proteins with blocked N termini
  • Internal amino acid sequence analysis after protease digestion in-gel and on-blot
  • Improved microscale peptide purification methods
  • Computer analysis of protein sequences
  • New protocols tested and refined through everyday use in authors' laboratories
  • Updated reference chapter covering all aspects of protein microsequencing

Review:

Reviews of the First Edition: "Like the evening network television news programs, this book gives a useful summary of the highlights (of protein chemistry) to enable the masses to go forth armed with enough information to survive in the world." --Cell

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Book Description Elsevier Science Publishing Co Inc, United States, 1993. Paperback. Book Condition: New. 2nd Revised edition. 226 x 152 mm. Language: English . Brand New Book. Why a Second Edition? The Second Edition provides practical answers to the general question, How can I obtain useful sequence information from my protein or peptide? rather than the more specific question asked in the first edition, How can I obtain the N-terminal sequence? Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry. Bookseller Inventory # AA59780124802827

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Book Description Elsevier Science Publishing Co Inc, United States, 1993. Paperback. Book Condition: New. 2nd Revised edition. 226 x 152 mm. Language: English . Brand New Book. Why a Second Edition? The Second Edition provides practical answers to the general question, How can I obtain useful sequence information from my protein or peptide? rather than the more specific question asked in the first edition, How can I obtain the N-terminal sequence? Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry. Bookseller Inventory # AA59780124802827

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