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A Practical Guide to Protein and Peptide Purification for Microsequencing - Softcover

 
9780124802827: A Practical Guide to Protein and Peptide Purification for Microsequencing
Softcover
ISBN 10: 0124802826 ISBN 13: 9780124802827
Publisher: Academic Press, 1993

Synopsis

Why a Second Edition?
The Second Edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" rather than the more specific question asked in the first edition, "How can I obtain the N-terminal sequence?" Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry.

  • Mass spectrophotometric characterization of proteins and peptides
  • N-terminal sequencing of proteins with blocked N termini
  • Internal amino acid sequence analysis after protease digestion in-gel and on-blot
  • Improved microscale peptide purification methods
  • Computer analysis of protein sequences
  • New protocols tested and refined through everyday use in authors' laboratories
  • Updated reference chapter covering all aspects of protein microsequencing

"synopsis" may belong to another edition of this title.

From the Back Cover

Why a Second Edition?
The second edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" rather than the more specific question asked in the first edition, "How can I obtain the N-terminal sequence?" Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry.
@introbul:Highlights of the Second Edition:
@bul:* Mass spectrophotometric characterization of proteins and peptides
* N-terminal sequencing of proteins with blocked N termini
* Internal amino acid sequence analysis after protease digestion in-gel and on-blot
* Improved microscale peptide purification methods
* Computer analysis of protein sequences
* New protocols tested and refined through everyday use in authors' laboratories
* Updated reference chapter covering all aspects of protein microsequencing

"About this title" may belong to another edition of this title.

Publisher
Academic Press
Publication date
1993
Language
English
ISBN 10 
0124802826
ISBN 13 
9780124802827
Binding
Plastic Comb
Edition number
2
Number of pages
184
Editor
Matsudaira Paul T.