A Practical Guide to Protein and Peptide Purification for Microsequencing - Softcover

Softcover

ISBN 10: 0124802826 ISBN 13: 9780124802827

Publisher: Academic Press, 1993

Synopsis

Why a Second Edition?
The Second Edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" rather than the more specific question asked in the first edition, "How can I obtain the N-terminal sequence?" Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry.

• Mass spectrophotometric characterization of proteins and peptides
• N-terminal sequencing of proteins with blocked N termini
• Internal amino acid sequence analysis after protease digestion in-gel and on-blot
• Improved microscale peptide purification methods
• Computer analysis of protein sequences
• New protocols tested and refined through everyday use in authors' laboratories
• Updated reference chapter covering all aspects of protein microsequencing

About this title

From the Back Cover:

Why a Second Edition?
The second edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" rather than the more specific question asked in the first edition, "How can I obtain the N-terminal sequence?" Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry.
Highlights of the Second Edition:
* Mass spectrophotometric characterization of proteins and peptides
* N-terminal sequencing of proteins with blocked N termini
* Internal amino acid sequence analysis after protease digestion in-gel and on-blot
* Improved microscale peptide purification methods
* Computer analysis of protein sequences
* New protocols tested and refined through everyday use in authors' laboratories
* Updated reference chapter covering all aspects of protein microsequencing

Review:

Reviews of the First Edition: "Like the evening network television news programs, this book gives a useful summary of the highlights (of protein chemistry) to enable the masses to go forth armed with enough information to survive in the world." --Cell

About this edition

• Publisher: Academic Press
• Publication date: 1993
• ISBN 10: 0124802826
• ISBN 13: 9780124802827
• Binding: Plastic Comb
• Edition number: 2
• Number of pages: 184
• Editor: Matsudaira Paul T.