Softcover

ISBN 10: 0127364501 ISBN 13: 9780127364506

Publisher: Academic Press, 1993

This volume provides, for the first time, an extensive compilation of those state-of-the-art methods that have revolutionized the use of mice as an experimental system. This volume is a unique source for detailed descriptions to be used in research by both experienced and novice researchers - Germ cells and embryos; Fertilization; Gene expression: messenger RNA, reporter genes, proteins, methylation; Gene identification; Nuclear transplantation; Transgenic animals: pronuclear injection, embryonic stem cells, and gene targeting; Lineage analysis.

Key Features
* Contains the most up-to-date methodology as applied to mouse development
* Focuses on molecular approaches to experimental mouse development
* The first comprehensive source for molecular biological methods in mouse development
* Many of the methods have never appeared before

From the Back Cover:

Guide to Techniques in Mouse Development , a volume designed to meet the needs of students, experienced researchers, and newcomers to the field, presents for the first time in a single source up-to-date technology used in research carried out with mice.
Key Features of the Guide
* Comprehensive molecular biological methods in mouse development compiled specifically for this volume
* Articles written by some of the most experienced researchers in the field of mouse development
Among the topics covered
* Gametes and embryos
* Transgenics
* Gene targeting
* Embryonic stem (ES) cells
* Microinjection
* Nuclear transplantation
* Reporter genes
* Gene cloning
* YAC libraries
*In situhybridization

Review:

Praise for the Volume
"Each section is divided into comprehensive subsections with introduction methodology, analysis of data. The text is clear and the methods are well presented in an itemized way. It even gives the source of the chemicals and how to store them. This book is highly recommended for anyone interested in molecular analysis of gene development in mice as well as for senior and even junior researchers operating in the general field of molecular biology."
--CELLULAR & MOLECULAR BIOLOGY
Praise for the Series
"The Methods in Enzymology series represents the gold-standard."
--NEUROSCIENCE
"Incomparably useful."
--ANALYTICAL BIOCHEMISTRY
"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page."
--BIO/TECHNOLOGY
"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection."
--CHEMISTRY IN INDUSTRY
"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced."
--AMERICAN SOCIETY OF MICROBIOLOGY NEWS
"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work."
--ENZYMOLOGIA
"A series that has established itself as a definitive reference for biochemists."
--JOURNAL OF CHROMATOGRAPHY