Understanding PCR: A Practical Bench-Top Guide - Softcover

Maddocks, Sarah; Jenkins PhD, Rowena

Softcover

ISBN 10: 0128026839 ISBN 13: 9780128026830

Publisher: Academic Press, 2016

Synopsis

Understanding PCR: A Practical Bench-Top Guide gives you all of the information you need to plan your first PCR, from reagents to conditions to analysis and beyond. It is a user friendly book that has step-by-step basic protocols, which can be adapted to your needs. Includes helpful information such as where to order your reagents and basic troubleshooting hints and tips.

• Includes resources for reagents
• Explains basic laboratory preparation
• Provides straightforward experimental protocols
• Incorporates fundamental analytical techniques
• Contains a troubleshooting guide

About this title

About the Authors

Sarah Maddocks, PhD, is a lecturer in Microbiology at Cardiff Metropolitan University, UK. Her research focuses on understanding the interactions between pathogens and their host during the infection process. The overarching aim of these studies is to understand how pathogens utilise or disrupt host processes and to explore and exploit novel ways of impairing these host-pathogen interactions to facilitate clearance of infection. Dr. Maddocks’ research also includes biofilm and co-culture models, genomic and molecular analysis of gene expression to prove clinically relevant hypotheses.

Rowena Jenkins, PhD, is currently a medical writer at Health Economics and Outcomes Research Ltd, UK. Prior to this she served as lecturer in Microbiology and Infectious Disease in the College of Medicine at Swansea University, UK. Her research team are focused on the effect of novel antimicrobial agents on pathogenic bacteria found in clinical infections, particularly chronic infections such as those seen in diabetic foot ulcers and cystic fibrosis lungs infection, and the impact on the microbiome within those conditions. Dr. Jenkins also has an interest in the potential for natural antimicrobial agents to improve the efficacy of conventional antibiotics against antibiotic resistant bacteria and biofilms. Dr. Jenkins serves as a section editor for the Journal of Medical Microbiology, and has co-authored two books with Elsevier, including the first edition of "Understanding PCR".

Edition details

• Publisher: Academic Press
• Publication date: 2016
• Language: English
• ISBN 10: 0128026839
• ISBN 13: 9780128026830
• Binding: Paperback
• Edition number: 1
• Number of pages: 98