Principles and Applications of Clinical Mass Spectrometry: Small Molecules, Peptides, and Pathogens - Softcover

Softcover

ISBN 10: 0128160632 ISBN 13: 9780128160633

Publisher: Elsevier, 2018

Synopsis

Principles and Applications of Clinical Mass Spectrometry: Small Molecules, Peptides, and Pathogens is a concise resource for quick implementation of mass spectrometry methods in clinical laboratory work. Focusing on the practical use of these techniques, the first half of the book covers principles of chromatographic separations, principles and types of mass spectrometers, and sample preparation for analysis; the second half outlines the main applications of this technology within clinical laboratory settings, including determination of small molecules and peptides, as well as pathogen identification. A thorough yet succinct guide to using mass spectrometry technology in the clinical laboratory, Principles and Applications of Clinical Mass Spectrometry: Small Molecules, Peptides, and Pathogens is an essential resource for chemists, pharmaceutical and biotech researchers, certain government agencies, and standardization groups.

• Provides concrete examples of the main applications of mass spectrometry technology
• Describes current capabilities of the LC- and MS-based analytical methods
• Details methods for successful analytical work in the field

About this title

About the Authors

Andrea Rita Horvath’s key research interests include evidence-based laboratory medicine (EBLM) including evidence-based monitoring, guideline development, and evaluation of new biomarkers and overdiagnosis. She holds advisory roles on test utilization and reimbursement policy at the National Prescribing Service of Australia and Medicare. She has published 160 research papers and 16 book chapters and has been an invited speaker at over 100 international conferences. Her national and international leadership positions include Chair of the IFCC Committee on EBLM (2003-2008); European Communities Confederation of Clinical Chemistry and Laboratory Medicine (EC4) Secretary (2005-2007); President of the Hungarian Society (2005-2008) and the Hungarian College of Laboratory Medicine (2008-2009); President-Elect (2007-2009), President (2009-2011) and Past President (2012-2013) of the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM); chair of the EFLM working group on Test Evaluation (2011-2015) and member of the Board of Directors of the American Association of Clinical Chemistry (2014-2016).

Andy Hoofnagle received his undergraduate education at Cornell University. He obtained his medical degree from the University of Colorado Health Sciences and his PhD from the University of Colorado, Boulder as part of the Medical Scientist Training Program. During his residency and chief residency in Clinical Pathology in the Department of Laboratory Medicine at the University of Washington, he did his post-doctoral work in the laboratory of Jay Heinecke, Department of Medicine. He was then promoted to Assistant Professor of Laboratory Medicine and now serves as the Director of Clinical Mass Spectrometry.
Dr. Hoofnagle’s laboratory focuses on using proteomic and metabolomic approaches to investigate the intersection of inflammation and lipid metabolism. His laboratory is pioneering the use of immunoaffinity peptide enrichment strategies of analyte-specific peptides from tryptic digests in the clinical analysis of low-abundance serum proteins. The laboratory has developed assays for small molecule analytes, including arginine, arginine metabolites, and vitamin D, which are being used in basic science and large-scale clinical studies. Several mouse models are being used to assess the importance of complement regulatory proteins in insulin sensitivity and lipid metabolism. All of these studies are in collaboration with outstanding investigators from the University of Washington and elsewhere.

From the Back Cover

Principles and Applications of Clinical Mass Spectrometry: Small Molecules, Peptides, and Pathogens - A Derivative of the Tietz Textbook of Clinical Chemistry and Molecular Diagnostics, 6th ed.

A concise resource for quickly implementing mass spectrometry methods in clinical laboratory work, derived from the sixth edition of the bestselling Tietz Textbook of Clinical Chemistry and Molecular Diagnostics.

Edited by Nader Rifai

Professor of Pathology, Harvard Medical School, Cambridge, MA USA

Director of Clinical Chemistry, Boston Children's Hospital, Boston, MA USA

Edited by Andrea Rita Horvath

Professor, School of Medical Sciences, University of New South Wales, Sydney, Australia

Edited by Carl T. Wittwer

Professor of Pathology, University of Utah School of Medicine, Salt Lake City, UT USA

Edited by Andy Hoofnagle

Associate Professor, Department of Laboratory Medicine, University of Washington, Seattle, WA USA

Derived from the sixth edition of the bestselling Tietz Textbook of Clinical Chemistry and Molecular Diagnostics, editors Nader Rifai, A. Rita Horvath, Carl T. Wittwer, and Andy Hoofnagle bring you Principles and Applications of Clinical Mass Spectrometry: Small Molecules, Peptides, and Pathogens. This is a concise resource for quick implementation of mass spectrometry methods for use in clinical laboratory work. Focusing on the practical use of these techniques, the book consists of two parts: part one covers principles of chromatographic separations, principles and types of mass spectrometers, and sample preparation for analysis; and part two provides guidance on the use of the technology in the main applications in the clinical lab, including determination of small molecules and peptides as well as in pathogen identification. For those who need a thorough yet succinct guide to using this technology in the lab, there is no better source.

• Provides concrete examples about the main applications of this technology
• Authored and edited by the top experts in the United States
• Not only describes the current capabilities of the LC- and MS-based analytical methods, but also provides the recipes for successful analytical work in this field

Related Elsevier Titles

• Makowski, Advances in Clinical Chemistry, vol. 79, February 2017, 9780128120767
• Nair & Clarke, Mass Spectrometry for the Clinical Laboratory, November 2016, 9780128008713
• Ghosh, Introduction to Protein Mass Spectrometry, December 2015, 9780128051238
• Vekey et al., Medical Applications of Mass Spectrometry, October 2007, 9780444519801

Edition details

• Publisher: Elsevier
• Publication date: 2018
• Language: English
• ISBN 10: 0128160632
• ISBN 13: 9780128160633
• Binding: Paperback
• Edition number: 1
• Number of pages: 216
• Editor: Rifai PhD Nader, Horvath A. Rita, Wittwer Carl T., Hoofnagle Andy