The Yeast Two-Hybrid System (Advances in Molecular Biology)

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9780195109382: The Yeast Two-Hybrid System (Advances in Molecular Biology)

Biologists have already identified thousands of new gene sequences, and genome sequencing efforts are speeding up the discovery process even further. With this explosion of sequence information comes the need to understand how genes work in concert in order to fulfill the cells functions. The yeast two-hybrid system--used to identify protein-protein interactions--is one of the most powerful and versatile methods for characterizing a protein's function. It has become an essential tool for both academic researchers and those in biotechnology and pharmaceutical companies. This volume presents work by pioneers in the field and is the first publication devoted solely to the yeast two-hybrid system. It includes detailed protocols, practical advice on troubleshooting, and suggestions for future development. In addition, it explains how to construct an activation domain hybrid library, how to identify mutations that disrupt an interaction, and how to use the system in mammalian cells. Many of the contributors have developed new applications and variations of the technique. Chapter topics include characterizing hormone/receptor complexes, identifying peptide ligands, analyzing interactions mediated by protein modifications, and dissecting the cell cycle and other complex genetic networks. The Yeast Two-Hybrid System is the single complete resource for scientists interested in this powerful research method.

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About the Author:

Paul L. Bartel is at Myriad Genetics, Salt Lake City. Stanley Fields is at University of Washington.

Review:


"Since the development of the yeast two-hybrid system by Fields and colleagues, it has developed into one of the most popular tools in biology. So much so that it has become difficult to follow the numerous modifications, improvements and multitudinous applications of the technique. The consolidation of all this information into a single volume, The Yeast Two-Hybrid System, will now make it much easier for interested researchers to come to grips with the technology." --Nature Structural Biology


"The Yeast Two-Hybrid System is the first book of its kind with comprehensive coverage on both principles and practice of this powerful genetic system. For beginners, the book can serve as a useful introduction to the technique. For those who are already using one version or another of the system, the book will help to broaden their perspective. It can be used as either a lab manual or a general reference book. Almost everything one needs to know about the yeast two-hybrid system can be found in this book." --Nature Structural Biology


"This book is intended for the wide range of biologists interested in using the 'Two-Hybrid System' to identify proteins that interact. . . . Part I contains 4 chapters that describe the most common variations of the two-hybrid system, complete with details about plasmid structures and yeast strain genotypes. There is sufficient information in these chapters for the average molecular biologist to implement the technique. . . . This book is a great resource for beginners as well as those who already use this technique, because each chapter contains its own methods section. This allows one to compare strategies that have evolved separately in different labs. Furthermore, the book was edited by Stanley Fields, the inventor of the Two-Hybrid System, and the chapters were written by the workers who helped refine the methodology. It is a useful guide written by successful practitioners."--The Quarterly Review of Biology


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