Bioseparations Science and Engineering (Topics in Chemical Engineering)

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9780195123401: Bioseparations Science and Engineering (Topics in Chemical Engineering)

Designed for undergraduates, graduate students, and industry practitioners, Bioseparations Science and Engineering fills a critical need in the field. Current, comprehensive, and concise, it covers bioseparations unit operations in greater depth than other texts on this topic. In each of the chapters, the authors use a consistent method of explaining unit operations, starting with a qualitative description noting the significance and general application of the unit operation. They then illustrate the scientific application of the operation, develop the required mathematical theory, and finally, describe the applications of the theory in engineering practice, with an emphasis on design and scaleup. Unique to this text is a chapter dedicated to bioseparations process design and economics, in which a process simular, SuperPro Designer® is used to analyze and evaluate the production of three important biological products. Other unique features include basic information about bioproducts and engineering analysis and a chapter with bioseparations laboratory exercises. Bioseparations Science and Engineering is ideal for students and professionals alike. Features


· Incorporates numerous example problems within the chapters


· Offers extensive sets of problems at the end of chapters


· Includes basic information about bioproducts


· Provides thorough coverage of analytical methods for bioproducts


· Uses the simulation software SuperPro Designer® to illustrate the analysis and evaluation of the production of citric acid, recombinant human insulin, and monoclonal antibodies


· Includes laboratory exercises that support text material


· Accompanied by a solutions manual available to instructors who adopt this text


· Supplemented by a website (www.biosep.ou.edu) with new problems and examples and links to useful databases and manufacturers of bioseparations equipment and supplies

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About the Author:

Roger G. Harrison is at University of Oklahoma. Paul Todd is at University of Colorado.

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Harrison, Roger G.; Todd, Paul W.; Rudge, Scott R.; Petrides, Demetri
Published by Oxford University Press (2002)
ISBN 10: 0195123409 ISBN 13: 9780195123401
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Harrison, Roger G.; Todd, Paul W.; Rudge, Scott R.; Petrides, Demetri
Published by Oxford University Press (2002)
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Book Description Oxford University Press, 2002. Book Condition: New. Brand New, Unread Copy in Perfect Condition. A+ Customer Service! Summary: Preface All chapters (except Chapter 12) end with the following sections: Summary, Nomenclature, Problems, and References. Preface Chapter 1. Introduction to Bioproducts and Bioseparations 1.1. Instructional Objectives 1.2. Broad Classification of Bioproducts 1.3. Small Biomolecules 1.3.1. Primary Metabolites 1.3.2. Secondary Metabolites 1.3.3. Summary of Small Biomolecules 1.4. Macromolecules: Proteins 1.4.1. Primary Structure 1.4.2. Secondary Structure 1.4.3. Tertiary Structure Example 1.1: Effect of a Reducing Agent on Protein Structure and Mobility 1.4.4. Quaternary Structure 1.4.5. Prosthetic Groups and Hybrid Molecules 1.4.6. Functions and Commercial Uses of Proteins 1.4.7. Stability of Proteins 1.4.8. Recombinant Protein Expression 1.5. Macromolecules: Nucleic Acids and Oligonucleotides 1.6. Macromolecules: Polysaccharides 1.7. Particulate Products 1.8. Introduction to Bioseparations: Engineering Analysis 1.8.1. Stages of Downstream Processing Example 1.2: Initial Selection of Purification Steps 1.8.2. Basic Principles of Engineering Analysis 1.8.3. Process and Product Quality 1.8.4. Criteria for Process Development 1.9. The Route to Market 1.9.1. The Chemical and Applications Range of the Bioproduct 1.9.2. Documentation of Pharmaceutical Bioproducts 1.9.3. GLP and cGMP 1.9.4. Formulation Chapter 2. Analytical Methods 2.1. Instructional Objectives 2.2. Specifications 2.3. Assay Attributes 2.3.1. Precision 2.3.2. Accuracy 2.3.3. Specificity 2.3.4. Linearity, Limit of Detection, and Limit of Quantitation 2.3.5. Range 2.3.6. Robustness 2.4. Analysis of Biological Activity 2.4.1. Animal Model Assays 2.4.2. Cell-Line-Derived Bioassays 2.4.3. In Vitro Biochemical Assays Example 2.1: Coupled Enzyme Assay for Alcohol Oxidase 2.5. Analysis of Purity 2.5.1. Electrophoretic Analysis Example 2.2: Estimation of the Maximum Temperature in an Electrophoresis Gel 2.5.2. High Performance Liquid Chromatography (HPLC) 2.5.3. Mass Spectrometry 2.5.4. Coupling of HPLC with Mass Spectrometry 2.5.5. UV Absorbance Example 2.3: Determination of Molar Absorptivity 2.5.6. CHNO/Amino Acid Analysis (AAA) Example 2.4: Calculations Based on CHNO Analysis 2.5.7. Protein Assays 2.5.8. Enzyme-Linked Immunosorbent Assay 2.5.9. Gas Chromatography 2.5.10. DNA Hybridization 2.5.11. ICP/MS (AA) 2.5.12. Dry Weight 2.6. Microbiology Assays 2.6.1. Sterility 2.6.2. Bioburden 2.6.3. Endotoxin 2.6.4. Virus and Phage Chapter 3. Cell Lysis and Flocculation 3.1. Instructional Objectives 3.2. Some Elements of Cell Structure 3.2.1. Prokaryotic Cells 3.2.2. Eukaryotic Cells 3.3. Cell Lysis 3.3.1. Osmotic and Chemical Cell Lysis 3.3.2. Mechanical Methods of Lysis 3.4. Flocculation 3.4.1. The Electric Double Layer Example 3.1: Dependence of the Debye Radius on the Type of Electrolyte 3.4.2. Forces between Particles and Flocculation by Electrolytes Example 3.2: Sensitivity of Critical Flocculation Concentration to Temperature and Counter-Ion Charge Number 3.4.3. The Schulze-Hardy Rule </br. Bookseller Inventory # ABE_book_new_0195123409

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Harrison, Roger G.; Todd, Paul W.; Rudge, Scott R.; Petrides, Demetri
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Harrison, Roger G.; Todd, Paul W.; Rudge, Scott R.; Petrides, Demetri
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Harrison, Roger G.; Todd, Paul W.; Rudge, Scott R.; Petrides, Demetri
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Book Description Oxford, 2002. Book Condition: Brand New. Brand New Hardcover Textbook is still wrapped MINT in the plastic. Ship from Multiple Locations, including Malaysia, Singapore, and Thailand. Shipping should take from 3-4 business days within US, Canada, UK, and other EU countries, 2-3 business days within Australia, Japan, and Singapore; for faster processing time, please choose to ship with Expedite. Thank you for looking![3401sOXBioseperScienEngine]. Bookseller Inventory # 978019512340188

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Harrison, Roger G.; Todd, Paul W.; Rudge, Scott R.; Petrides, Demetri
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Book Description Oxford, 2002. Book Condition: Brand New. Brand New Hardcover Textbook is still wrapped MINT in the plastic. Ship from Multiple Locations, including Malaysia, Singapore, and Thailand. Shipping should take from 3-4 business days within US, Canada, UK, and other EU countries, 2-3 business days within Australia, Japan, and Singapore; for faster processing time, please choose to ship with Expedite. Thank you for looking![3401sOXBioseperScienEngine]. Bookseller Inventory # 978019512340188

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