The first book of a two volume treatise on protein purification methods and applications, this title covers both traditional and recently developed techniques for initial purification, which is fundamental to any investigation of protein chemistry. The theory and basic principles of each technique are discussed, including initial planning, clarification and extraction, concentration of the extract, structure-based separation, activity-based purification, and gel permeation chromatography. Problem areas and solutions are highlighted at each stage, making this book equally valuable to novice researchers and more experienced protein biochemists who will find it a reliable and readily accessible bench manual.
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S. Angal, both at Celltech Limited, Slough.
'beautifully presented ... a veritable mine of information from cover to cover ... One can only admire the professionalism of the editing and the technical expertise housed within ... standard of writing and editing is high'
Bob Ford, Chemistry and Industry, 2 September 1991
'It is useful bench manual and an accessible reference source recommended to a wide range of protein biochemists and molecular biologists, from novices to experienced protein purifiers.'
Mercedes G. Garaita and John F. Kennedy, University of Birmingham, The Genetic Engineer and Biotechnologist, September/October 1992
`I recommend this book as an excellent reference for the practical scientist about to enter the field of protein purification, as well as providing additional information for researchers already in the field.' Trends in Biotechnology
`a wel[l]come addition to the protein chemists' armoury, since it provides a wealth of practical detail'
`the index is comprehensive.' FEBS Letters
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