Items related to Protein Engineering: A Practical Approach (The ^APractical...
Synopsis
Protein engineering is the rational modification or redesign of proteins using genetic engineering. Thus, it is now possible to modify enzyme specifities, remodel antibodies, and redesign many multi-domain proteins for therapeutic purposes. While the procedures for the introduction of mutations have become routine, predicting and understanding the effects of these mutations can be complicated. This volume provides a comprehensive guide to the methods used at every stage of the engineering process, from the choice of mutation strategy, through protein stability studies, to critical evaluations of mammalian, yeast, and bacterial host expression systems. It is a practical guide to this fascinating mixture of molecular biology, protein structure analysis, computation and biochemistry.
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Review
'the first practical guide to this fascinating area of science ... the major parts of the book are very well written and of great didactic value ... The book is recommended for undergraduate and graduate students and to researchers in the field of protein engineering.' Arvind M. Kayastha, Biochemical Education 21(4) 1993 'The problem is to produce a specific purified protein in significant quantities. This book provides some practical details that can help in this task.' International Journal of Biochemistry, Vol. 25, No. 12, 1993 'Protein Engineering: A Practical Approach is the first reference book in this network of molecular biology, structure of proteins and biochemistry. It combines theretical background and detailed procedures; both together enable researchers in this field to plan and carry out experiments for their own projects. This book should therefore serve as the standard text for students and scientists in the field of protein engineering.' Arzneimittel-Forschung/Drug Research Issue 12/93
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