The subcellular distribution of proteins throughout eukaryotic cells is highly ordered. Thus proteins have to be transported to their correct destinations and left there. Understanding the complex mechanisms by which this occurs has provided a major challenge for modern biology. Recent progress in the subject has relied heavily on developing methods to measure protein transport and targeting. This detailed work presents contributions from active researchers in the field, providing step-by-step protocols for studying the processes involved, using both intact cells and broken cell preparations. Cell biological, biochemical, and molecular biological methods are all fully discussed in this book, and the chapters cover the well-established techniques and a number of exciting new methodologies which have wide applications. This book will enable researchers in cell biology, biochemistry, molecular biology, and physiology to unravel the mechanisms which control cellular protein targeting.
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Anthony I. Magee, Senior Scientist, National Institute for Medical Research, London. Thomas Wileman, Assistant Professor, Harvard medical School, Division of Immunology, Beth Israel Hospital, Boston, USA.
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