Signal Transduction: A Practical Approach (The ^APractical Approach Series) - Softcover

Softcover

ISBN 10: 0199632952 ISBN 13: 9780199632954

Publisher: Oxford University Press, 1992

Synopsis

Cellular trans-membrane signalling mechanisms are among the most highly studied processes in the biological sciences. G-proteins provide transduction components to allow communication between receptors for hormones and neurotransmitters and their effector systems which include enzymes involved in the generation of intracellular secondary messengers and a variety of ion channels. Signal Transduction: A Practical Approach details the experimental approaches which are used to define the contacts between receptors and G-proteins and between these G-proteins and their effector systems. While much emphasis in the cellular signalling field remains on the measurement of adenylyl cyclase and of phosphoinositidase C activities, it is now clear that activation of G-protein linked receptors can also cause the regulation of phospholipases of the D and A2 classes. Thus, assays used to measure the activities of these enzyme systems are detailed in this book. The use of mutagenesis to define functional domains of G-proteins themselves has undergone considerable progress recently and examples of this approach are also included. This volume provides a useful guide to the newcomer to the subject, as well as allowing workers already in the field to expand the range of approaches in use in their laboratories.

About this title

About the Author

G. Milligan, Department of Biochemistry, University of Glasgow.

Review

'This volume provides a useful guide ... As is usual in this series, protocols are set out in some detail with full discussion of practical difficulties and limitations, and the book can be used by both newcomers to the field and experienced laboratory workers.'
Aslib Book Guide, Vol. 58, No. 1, January 1993

'an extremely useful addition to the collection of anyone interested in studying signal transduction ... ideal for researchers new to the area or researchers wishing to expand the range of intracellular messengers investigated in their laboratory'
Cardiovascular Research, April 1994

Edition details

• Publisher: Oxford University Press
• Publication date: 1992
• Language: English
• ISBN 10: 0199632952
• ISBN 13: 9780199632954
• Binding: Paperback
• Number of pages: 206
• Editor: Milligan G.