PCR 2: A Practical Approach (The ^APractical Approach Series) - Hardcover

Hardcover

ISBN 10: 0199634254 ISBN 13: 9780199634255

Publisher: Oxford University Press, 1995

Synopsis

PCR is now an indispensable tool for the molecular biologist, heralding an era of unparalleled research productivity. The basic PCR technique provides the cornerstone of in vitro DNA amplification, upon which further features can be built to expand the range of PCR-based applications.
PCR 2: A Practical Approach is not a revised version of PCR: A Practical Approach, but sets out to address some of the exciting new applications of PCR and so it is complementary to the original volume. The book starts with an essential guide for improving PCRs, then deals with the use of modified primers and the direct sequencing of PCR products. The focus then switches to mRNA and covers cDNA cloning, mRNA quantitation, and direct expression of proteins from PCR products. Finally genomic DNA analysis is addressed including genome mapping and fingerprinting, the analysis of mutations, and the direct sequencing and analysis of methylation patterns. The book is essential reading for all researchers in molecular biology and related disciplines.

About this title

About the Author

B. D. Hames, Department of Biochemistry, both at the University of Leeds. G.R. Taylor, Regional DNA Laboratory, Leeds.

Review

`This book gives a good insight into the more advanced PCR technology avaiable and will certainly be a useful addition to laboratories wishing to expand their PCR repertoire.'
Dr Amanda Evans, Pharma Research, CIBA AG, Basel, Cell Biology International, 1996, Vol. 20, No. 6

Edition details

• Publisher: Oxford University Press
• Publication date: 1995
• Language: English
• ISBN 10: 0199634254
• ISBN 13: 9780199634255
• Binding: Hardcover
• Number of pages: 358
• Editor: McPherson M. J., Hames B. D., Taylor G. R.