Plasmids: A Practical Approach (Practical Approach Series) - Softcover

Softcover

ISBN 10: 0199634440 ISBN 13: 9780199634446

Publisher: IRL Press, 1994

Synopsis

This completely updated and revised second edition of Plasmids: A Practical Approach provides the researcher with detailed protocols for studying bacterial plasmids and for using plasmids and phagemids as vectors. With many new sections and protocols, it is the most up-to-date reference describing the techniques for the study of plasmid replication and maintenance, the fundamental methods for purifying plasmids and introducing them into cells, and the protocols for the mutagenesis and analysis of plasmid-encoded products. The book features further techniques for the study of plasmids and the use of plasmid vectors in such important groups of bacteria as animal and plant pathogens harboring virulence plasmids, Streptomyces, and lactococci. Plus, the book describes the use of plasmid composite vectors for expression of cDNA cloning, and much more. It is a invaluable reference for molecular biologists, microbiologists, and biotechnology researchers. It includes detailed protocols for studying bacterial plasmids and for using both plasmids and phagemids as vectors, techniques for studying fundamental aspects of plasmids, such as replication, are included, as well as methods for using plasmid vectors in important groups of bacteria. Also described are protocols for using phagemids, which are extremely useful tools for genetic engineering.

About this title

About the Author

K. G. Hardy, Assistant Director, Glaxo Institute for Molecular Biology, Geneva.

Edition details

• Publisher: IRL Press
• Publication date: 1994
• Language: English
• ISBN 10: 0199634440
• ISBN 13: 9780199634446
• Binding: Paperback
• Edition number: 2
• Number of pages: 272
• Editor: Hardy K. G.