Peptide Antigens: A Practical Approach (The ^APractical Approach Series) - Hardcover

Hardcover

ISBN 10: 0199634521 ISBN 13: 9780199634521

Publisher: Oxford University Press, 1995

Synopsis

Peptide antigens and anti-peptide antibodies are widely used in biochemistry and molecular biology for the measurement, location, and purification of specific oligopeptides. More recently the application of these reagents has expanded, for example in the identification and mapping of the binding sites of antibodies and T-cell receptors and in the identification and characterization of proteins which are known only by their primary structure. This volume provides practical guidance to the major techniques used in the exploitation of peptide antigens and anti-peptide antibodies. The chapters give detailed protocols for the prediction of epitopes, peptides synthesis, the preparation of peptide immunogens, immunoaffinity chromatography, immunoassays, and the mapping of epitopes using both synthetic peptides and phage display systems.
Peptide Antigens: A Practical Approach covers all practical aspects of this important and growing subject. It is a unique compendium of methods for workers in biochemistry, molecular biology, and immunology who need to use this technology in their research.

About this title

About the Author

G. Brian Wisdom, Division of Biochemistry, Medical Biology Centre, The Queen's University, Belfast.

Review

`a valuable addition to the Practical Approach series ... a very handy compendium of protocols for anyone working with peptides in immunology'
European Peptide Society Newsletter

`provides practical guidance to the major techniques used in the exploitation of peptide antigens and anti-peptide antibodies ... immunoaffinity chromatography, immunoassays, and the mapping of epitopes'
Biofutur - Mai 1995

`This volume is another fine addition to The Practical Approach Series ... the book lives up to the consistently high quality of this series ... useful small volume which deserves to become a good companion to the scientist at the bench.'
Hans Rudolf Bosshard, FEBS Letters 369 (1995)

Edition details

• Publisher: Oxford University Press
• Publication date: 1995
• Language: English
• ISBN 10: 0199634521
• ISBN 13: 9780199634521
• Binding: Hardcover
• Edition number: 1
• Number of pages: 272
• Editor: Wisdom G. Brian