Synopsis
A flow cytometer measures physical and chemical characteristics of single cells or organelles and enables them to be identified, counted, and physically separated (sorted) for further study. Flow cytometry is little more than 20 years old and is still unfamiliar to many biologists. The rapid increase in the number of machines being installed has meant that there are many comparatively inexperienced workers trying to cope with the new technology. This book offers practical guidance in preparing and staining their samples. It should also enable more experienced practitioners who want to investigate a new application. This second edition has been fully updated to reflect new developments. For example chapters on fluorescence technology, DNA analysis and the measurement of cell proliferation, chromosome analysis and sorting, membrane potential and pH, and the further applications to cell biology, have been updated and, where necessary, expanded. New authors have written enlarged chapters on immunofluorescence of surface markers and measurement of cytoplasmic and nuclear antigens.
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Review
'this is a useful book for beginners who have an interest in applying flow cytometry to their particular area of biology' Richard S. Camplejohn, St Thomas' Hospital, BioEssays, Vol.14, No.1 - January 1992
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Flow Cytometry: A Practical Approach
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