RNA Processing: A Practical ApproachVolume II (The ^APractical Approach Series) - Hardcover

Hardcover

ISBN 10: 0199634718 ISBN 13: 9780199634712

Publisher: Oxford University Press, 1994

Synopsis

Post-transcriptional events are increasingly recognized as important sites of regulation of gene expression in eukaryotic cells and so RNA processing has rapidly become an area of intense research activity. These two volumes give detailed practical guidance on all the major aspects of this subject. Step-by-step practical protocols from leading laboratories are presented for studies of the termination and end-processing, capping, methylation, splicing, and editing of mRNA as well as studies of mRNA stability and processing of rRNA and tRNA. The reader is led through all the key steps required for a successful experimental investigation. This includes full descriptions of the synthesis and purification of RNA substrates for in vitro work, the characterization of specific RNAs, and the isolation and analysis of ribonucleoprotein complexes. RNA processing - A Practical Approach, Volumes I and II, are invaluable laboratory companions for researchers working on mRNA, rRNA, or tRNA gene expression. Their lucid explanatory style and comprehensive coverage of all the key techniques for analysing RNA processing will be welcomed by both experienced researchers and

About this title

About the Author

Stephen J. Higgins and B. David Hames are both at the University of Leeds.

Edition details

• Publisher: Oxford University Press
• Publication date: 1994
• Language: English
• ISBN 10: 0199634718
• ISBN 13: 9780199634712
• Binding: Hardcover
• Number of pages: 278
• Editor: Higgins Stephen J., Hames B. David