Bioenergetics: A Practical Approach - Softcover

Softcover

ISBN 10: 0199634882 ISBN 13: 9780199634880

Publisher: IRL Press, 1996

Synopsis

Bioenergetics is a topic which impinges on a wide range of fundamental problems in biology. The movement of electrons and ions across and through membranes is important in fields such as energy transduction, cell biology, and cell signalling. The maturation of bioenergetics has led to cross-fertilization with other fields and this has led to both the necessity for scientists in other areas, such as clinical scientists, to learn the classical bioenergetic techniques, and the application of foreign techniques to bioenergetics, like molecular biology and electrophysiology.
This book describes the core bioenergetic techniques required in the analysis of membrane-translocating events. Topics covered include the measurement of the main fluxes and stoichiometries, membrane transport, redox states and potentials, the isolation of bioenergetic preparations for mitochondria and mitochondrial subcomponents and chloroplast subcomponents, and the reconstitution and characterization of proteins in proteoliposomes. Both established and new techniques are fully described. The book will be a valuable guide for research scientists working in the areas of mammalian and plant biochemistry and cell biology.

About this title

About the Author

G. C. Brown is at University of Cambridge. C. E. Cooper is at University College of London Medical School.

From the Back Cover

The movement of electrons, protons, and other ions across and through membranes is crucially important in many fields including energy transduction, cell biology, and cell signalling. This book brings together the key methods and approaches of modern bioenergetics, to assist the researcher to probe and understand these phenomena. Topics covered include: measurement of oxygen, proton, and phosphate fluxes and stoichiometries; membrane permeability and transport; reconstitution of bioenergetic proteins and use of proteoliposomes; protonmotive force; redox states and potentials; control and regulation; patch clamping; studies of cellular energetics using (superscript 31)P-NMR; isolation and characterization of photosynthetic reaction centres in eukaryotes. Bioenergetics: A Practical Approach will be invaluable to established researchers as well as those who need to use these techniques for the first time. It is a comprehensive practical manual providing not only up-to-date laboratory protocols, but also many hints and tips for success, troubleshooting comments, and the essential theory behind important experimental approaches.

Edition details

• Publisher: IRL Press
• Publication date: 1996
• Language: English
• ISBN 10: 0199634882
• ISBN 13: 9780199634880
• Binding: Paperback
• Edition number: 1
• Number of pages: 224
• Editor: Brown Guy C., Cooper Chris E.