Platelets: A Practical Approach (The ^APractical Approach Series) - Hardcover

Watson, Authi

Hardcover

ISBN 10: 0199635382 ISBN 13: 9780199635382

Publisher: Oxford University Press, USA, 1996

Synopsis

Platelets are small blood cells that play a vital role in blood clotting. They are essential for wound healing, but their unwanted action can lead to ailments such as stroke and heart attack. In seventeen chapters from leading experts in platelet research this book describes state-of-the-art techniques for the experienced researcher, both in academia and industry. Methodology is also included that will enable the newcomer to perform routine experiments. Techniques covered range from basic procedures such as platelet preparation to the latest techniques such as monitoring calcium levels in single cells, protein tyrosine phosphorylation, and PCR.

About this title

From the Back Cover

Platelets play an essential role in blood clotting at sites of wound injury, but unwanted activation of platelets in the circulation can give rise to thrombus formation, and is implicated in the onset of stroke, myocardial infarction, and other diseases. Platelets are also one of the major model systems for the study of signal transduction. This book is a comprehensive and up-to-date practical guide to research on platelets. Each chapter, written by experts in the field, contains detailed protocols, illustrative results, tips and hints for success, and key literature citations. Topics covered include: platelet preparation; monitoring platelet aggregation and secretion; megakaryocyte differentiation; preparation and uses of semipermeabilized platelets; monitoring calcium; identification and characterization of platelet glycoproteins (including the use of PCR); analysis of cell-surface antigens; the role of the cytoskeleton in platelet function; measurement of protein serine/threonine and tyrosine phosphorylation, kinase activity, cyclic nucleotides and G protein function; analysis of phospholipid metabolism, immunocytochemistry and electron microscopy studies; clinical aspects of platelet function; platelet procoagulant activity; and studies of platelet function in species other than man.

About the Author

Stephen Watson is at University of Oxford. Kalwant Authi is at Thrombosis Research Institute, London.

Edition details

• Publisher: Oxford University Press, USA
• Publication date: 1996
• ISBN 10: 0199635382
• ISBN 13: 9780199635382
• Binding: Hardcover
• Language: English
• Number of pages: 400