Affinity Separations: A Practical Approach (The ^APractical Approach Series) - Hardcover

Hardcover

ISBN 10: 019963551X ISBN 13: 9780199635511

Publisher: Oxford University Press, 1997

Synopsis

Affinity Separations: A Practical Approach is a new collection of affinity-based purification protocols with an international authorship from across the life sciences. The introductory chapter outlines recent advances in ligand-coupling, matrix choice, and operating modes. The second chapter describes methods for obtaining quantitative data from affinity processes. There are specific chapters describing methods for the affinity separation of proteins, nucleic acids, and oligosaccharides. Methods for immunoglobulin purification and immunoaffinity protocols are discussed in a further chapter, and the use of affinity tags in the recovery of engineered proteins in another. Finally, modern techniques for cell separations are outlined. The book presents detailed laboratory protocols together with troubleshooting advice and reviews of recent developments. It is aimed at bench scientists, both those in analytical research laboratories, and those non-specialists looking to apply affinity methods in their work.

About this title

About the Author

Paul Matejtschuk, Doctor, Research and Development, BioProducts Laboratory, Hertfordshire.

Edition details

• Publisher: Oxford University Press
• Publication date: 1997
• Language: English
• ISBN 10: 019963551X
• ISBN 13: 9780199635511
• Binding: Hardcover
• Number of pages: 272
• Editor: Matejtschuk Paul