Cell Separation: A Practical Approach (The ^APractical Approach Series) - Hardcover

Hardcover

ISBN 10: 0199635803 ISBN 13: 9780199635801

Publisher: Oxford University Press, 1999

Synopsis

Techniques for separating cells are needed in many areas of cell biology. This book presents modern methods from the laboratories of experts in the field, and includes tested, reproducible protocols, hints and tips for success, and troubleshooting suggestions. It will be invaluable to a wide
range of cell biologists.

About this title

About the Author

Derek Fisher, Molecular Cell Pathology, Royal Free Hospital School of Medicine. Gillian E. Francis, Molecular Cell Pathology, Royal Free Hospital School of Medicine. David Rickwood, Department of Biology, University of Essex.

Review

"In this reference British researchers have contributed articles covering the process of cell separation using a number of different methods, with explanations of the theoretical bases for these procedures. The chapters explain when a particular method would be best used, based on cell size, density, surface charge, hydrophobic surface properties, and antigen status. The methods described include sedimentation, centrifugal elutriation, partitioning in aqueous two-phase systems, flow cytometry, immuno methods (including magnetic, column, and panning techniques), and free flow electrophoresis."--SciTech Book News

"The title of this book describes the contents within, and reviews a subject that is underrepresented in the literature. The seven chapters discuss the preparation of single cell suspensions and six typical separation methods: sedimentation, centrifugal elutriation, partitioning in aqueous two-phase systems, flow cytometry, immunomethods (magnetic, column, and panning techniques), and free flow electrophoresis. The descriptions emphasize practical applications of these techniques including protocols for most, if not all methods. Although the theoretical basis of these techniques are not heavily emphasized, adequate references are given for those interested."--The Quarterly Review of Biology

Edition details

• Publisher: Oxford University Press
• Publication date: 1999
• ISBN 10: 0199635803
• ISBN 13: 9780199635801
• Binding: Hardcover
• Language: English
• Number of pages: 286
• Editor: Fisher Derek, Francis Gillian E., Rickwood David