Antibody Engineering: A Practical Approach (The ^APractical Approach Series) - Hardcover

Hardcover

ISBN 10: 0199635935 ISBN 13: 9780199635931

Publisher: Oxford University Press, 1996

Synopsis

Recombinant DNA techniques have revolutionized the isolation and production of antibodies. This volume describes methods and technologies which will allow the researchers to isolate a new antibody, analyse its properties, format the correct antibody or fragment, and produce sufficient quantities for experimental use.
Antibody Engineering is a unique manual of recombinant DNA methods for all those working with antibodies in research, diagnostics, and therapeutics. Each chapter is written by a leading researcher in the field and provides essential background information, fully tested protocols, sample data from using these methods, trouble-shooting comments, and key hints and tips for success.

About this title

About the Author

David J. Chiswell, all at Cambridge Antibody Technology Limited.

From the Back Cover

Recombinant DNA techniques have revolutionized the isolation and production of antibodies. This volume describes methods and technologies which will allow the researcher to isolate a new antibody, analyse its properties, format the correct antibody or fragment, and produce sufficient quantities for experimental use. Topics in this volume include: generation and analysis of antibodies; antibody repertoires; antibody screening and selection; measuring antibody affinities; sequence analysis; antibody engineering and production; conversion of rodent antibodies to human antibodies by CDR grafting or guided selection; choosing and optimizing effector functions; preparation and use of antibody-based molecules in eukaryotic and prokaryotic systems; scaling up manufacture; and generation of high-affinity high-specificity human antibodies in appropriate formats. Antibody Engineering is a unique manual of recombinant DNA methods for all those working with antibodies in research, diagnostics, and therapeutics. Each chapter is written by a leading researcher in the field and provides essential background information, fully tested protocols, sample data from using these methods, trouble-shooting comments, and key hints and tips for success.

Edition details

• Publisher: Oxford University Press
• Publication date: 1996
• Language: English
• ISBN 10: 0199635935
• ISBN 13: 9780199635931
• Binding: Hardcover
• Number of pages: 348
• Editor: McCafferty John, Hoogenboom Hennie R., Chiswell David J.