Protein Function: A Practical Approach (The ^APractical Approach Series) - Hardcover

Hardcover

ISBN 10: 0199636168 ISBN 13: 9780199636167

Publisher: Oxford University Press, 1997

Synopsis

The analysis of protein function is a vital step in the characterization of any newly discovered protein. This new edition brings up to date the techniques used, and presents experimental procedures that can be performed in the average laboratory without recourse to highly specialised equipment. The protocols will be of use to both experienced and novice researchers and are accompanied by background information, hints and tips, and troubleshooting guides to ensure successful elucidation of protein function.

About this title

About the Author

T. E. Creighton, European Molecular Biology Laboratory, Heidelberg.

Review

"This edition . . . has been updated from the original 1989 edition . . . [N]ew chapters have . . . been added to reflect recent improvements in the techniques of molecular biology. . . . The ten chapters roughly focus on five areas. One of the main topics is the chemical modification of proteins . . . Another section considers techniques involved in the detection and quantification of ligand-receptor binding interactions. A third focuses on the purification and analysis of sequence-specific DNA binding proteins. . . . One of the new additions is a discussion of the techniques involved in the overexpression of cloned genes in E. coli and of those used in protein refolding. These chapters are particularly useful, as they contain warnings of numerous pitfalls as well as valuable suggestions that would aid researchers new to these areas. A final addition is a chapter on the identification of proteins by using two-dimensional electrophoresis."--The Quarterly Review of Biology

Edition details

• Publisher: Oxford University Press
• Publication date: 1997
• Language: English
• ISBN 10: 0199636168
• ISBN 13: 9780199636167
• Binding: Hardcover
• Edition number: 2
• Number of pages: 356
• Editor: Creighton T. E.