Protein Expression: A Practical Approach (Practical Approach Series)

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9780199636235: Protein Expression: A Practical Approach (Practical Approach Series)

Part of a mini-series on the synthesis and processing of proteins, this volume details the expression of cloned DNA and RNA templates in all of the major in vivo and in vitro systems. The in vivo systems covered are cultured mammalian cells, the yeasts Sacchoromyces cerevisiae and Pichia pastoris, baculovirus, Xenopus oocytes, and prokaryotic cells. It also covers cell-free systems of both eukaryotes and prokaryotes, including prokaryotic systems that offer coupled transcription-translation. The volume concludes with a chapter on monitoring protein expression.

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David Hames is at University of Leeds. Steve Higgins is at University of Leeds.

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"A laboratory manual compiling methods for monitoring protein expression in all the major in vivo and in vitro eukaryotic systems, including cultured mammalian cells, yeasts, vaculovirus, and Xenopus oocytes and also in prokaryotes in cell-free systems that allow coupled transcription-translation. The step-by-step protocols have been prepared by experienced researchers for graduate students, beginning researchers, and senior scientists."--SciTech Book News


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