Protein Expression: A Practical Approach (The ^APractical Approach Series) - Hardcover

9780199636242: Protein Expression: A Practical Approach (The ^APractical Approach Series)

Hardcover

ISBN 10: 0199636249 ISBN 13: 9780199636242

Publisher: Oxford University Press, 1999

Part of a mini-series on the synthesis and processing of proteins, this volume details the expression of cloned DNA and RNA templates in all of the major in vivo and in vitro systems. The in vivo systems covered are cultured mammalian cells, the yeasts Sacchoromyces cerevisiae and Pichia pastoris, baculovirus, Xenopus oocytes, and prokaryotic cells. It also covers cell-free systems of both eukaryotes and prokaryotes, including prokaryotic systems that offer coupled transcription-translation. The volume concludes with a chapter on monitoring protein expression.

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About the Author

David Hames, School of Biochemistry and Molecular Biology, University of Leeds. Steve Higgins, School of Biochemistry and Molecular Biology, University of Leeds.

Review

"A laboratory manual compiling methods for monitoring protein expression in all the major in vivo and in vitro eukaryotic systems, including cultured mammalian cells, yeasts, vaculovirus, and Xenopus oocytes and also in prokaryotes in cell-free systems that allow coupled transcription-translation. The step-by-step protocols have been prepared by experienced researchers for graduate students, beginning researchers, and senior scientists."--SciTech Book News

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