PCR 3: PCR In Situ Hybridization: A Practical Approach (Practical Approach Series) - Softcover

Softcover

ISBN 10: 019963632X ISBN 13: 9780199636327

Publisher: Oxford University Press, 1998

Synopsis

PCR 3: In Situ Hybridization is a unique and timely collection of well-tested protocols for the amplification of DNA and RNA in cells and tissues. For each topic covered, leading experts in the field provide detailed guidance on the key steps in the protocols, numerous hints and tips for success, and advice on trouble-shooting. Topics include: DNA in situ PCR and DNA PCR in situ hybridization (PCR ISH) for the detection of DNA targets in cells; reverse trancriptase in situ PCR (RT-PCR) and RT-PCR ISH for the detection of RNA targets; and PRINS (primed in situ synthesis) for chromosomal analysis in interphase nuclei and metaphase chromosome spreads. This book will be invaluable to molecular biologists, pathologists, geneticists, and all those seeking to perform in situ analyses of nucleic acid molecules.

About this title

About the Author

C. S. Herrington is at University of Liverpool. J. J. O'Leary is at John Radcliffe Hospital, Oxford.

Review

"The combination of the polymerase chain reaction (PCR) with in situ hybridization creates a powerful new technique that combines the best of both methods, enhances the sensitivity of in situ hybridization, and allows the morphological localization of the products of the PRC reaction. This volume. . .provides detailed protocols for the use of polymerase chain reaction on tissues, interphase cells, and metaphase chromosomes. 4 Stars."--Doody's Journal

"Polymerase chain reaction (PCR) in situ hybridization (ISH) is a hybrid technique . . . The conventional use of ISH allows cellular detection and localization of DNA and RNA by hybridization with complementary nucleic acids probes and, thus, helps in understanding the disease process. PCR, on the other hand, allows the detection of minute nucleic acids sequences, but it does not permit morphological correlation . . . The combination of both PCR and ISH has greatly enhanced the morphological localization of specific nucleic acids sequence in cells and tissues. . . . Overall, this book covers all aspects of the technical details regarding PCR in situ hybridization and it will find wide use in those laboratories who perform or intend to perform in situ analyses of nucleic acids. The editors ought to be complimented for putting together the state of the art techniques in one book, which may serve as a useful source in this technical field for a long time."--American Journal of Human Biology

Edition details

• Publisher: Oxford University Press
• Publication date: 1998
• ISBN 10: 019963632X
• ISBN 13: 9780199636327
• Binding: Paperback
• Language: English
• Edition number: 1
• Number of pages: 224
• Editor: Herrington C. Simon, O'Leary John J.