Items related to PCR 3: PCR In Situ Hybridization: A Practical Approach
Synopsis
PCR 3: In Situ Hybridization is a unique and timely collection of well-tested protocols for the amplification of DNA and RNA in cells and tissues. For each topic covered, leading experts in the field provide detailed guidance on the key steps in the protocols, numerous hints and tips for success, and advice on trouble-shooting. Topics include: DNA in situ PCR and DNA PCR in situ hybridization (PCR ISH) for the detection of DNA targets in cells; reverse trancriptase in situ PCR (RT-PCR) and RT-PCR ISH for the detection of RNA targets; and PRINS (primed in situ synthesis) for chromosomal analysis in interphase nuclei and metaphase chromosome spreads. This book will be invaluable to molecular biologists, pathologists, geneticists, and all those seeking to perform in situ analyses of nucleic acid molecules.
"synopsis" may belong to another edition of this title.
About the Author
C. S. Herrington, Senior Lecturer, University of Liverpool. J. J. O'Leary, Doctor, John Radcliffe Hospital, Oxford.
Review
`The purpose of this multiauthor manual is to provide protocols for in-cell amplification and detection of DNA and RNA in cells. Its 9 chapters cover (1) fixation of tissues for the polymerase chain reaction, (2) in situ genetic analysis with selective ultraviolte radiation fractionation, (3)
in situ hybridization, (4) plymerase chain reaciton in situ hybridization, (5) reverse transcriptase in situ polymerase chain reaction for RNA detection (6) primed in situ DNA syntehsis, (7) application of in situ polymerase chain reaction techniques to human tisseus, (8) applicationa dn
modifications of plymerase chain reaction in situ hybridization, and (9) automation of in situ amplification. There is a subject index, and a list of suppliers of material and equipment.'
CAB Abstracts
`'...These techniques have enormous ptential for biologists, pathologists, geneticists, etc. in allowing ready access to any DNA or RNA target within the cell and offer the investigator the ability to perform sub-genomic in situ analyses.''
Biochimica Clinica, 1999, vol. 23, n. 1
"About this title" may belong to another edition of this title.
- PublisherOxford University Press
- Publication date1998
- ISBN 10 0199636338
- ISBN 13 9780199636334
- BindingHardcover
- LanguageEnglish
- Edition number1
- Number of pages205
- EditorHerrington C. Simon, O'Leary John J.
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PCR 3: PCR In Situ Hybridization - A Practical Approach
Published by
IRL Press at Oxford University Press, Oxford, New York, and Tokyo, 1998
ISBN 10: 0199636338
ISBN 13: 9780199636334
Used
Hardcover
First Edition
Seller: Vashon Island Books, Vashon, WA, U.S.A.
Seller rating 5 out of 5 stars
Hardcover. Condition: Near Fine. No Jacket. First Edition. Size: 8vo - over 7¾" - 9¾" tall. Book. Seller Inventory # G8557
Quantity: 1 available
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Pcr 3: Pcr in Situ Hybridization : A Practical Approach (Practical Approach Series)
Published by
Oxford University Press, 1998
ISBN 10: 0199636338
ISBN 13: 9780199636334
Used
Hardcover
Seller: medimops, Berlin, Germany
Seller rating 5 out of 5 stars
Condition: very good. Gut/Very good: Buch bzw. Schutzumschlag mit wenigen Gebrauchsspuren an Einband, Schutzumschlag oder Seiten. / Describes a book or dust jacket that does show some signs of wear on either the binding, dust jacket or pages. Seller Inventory # M00199636338-V
Quantity: 1 available