High Resolution Chromatography: A Practical Approach (Practical Approach Series)

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9780199636495: High Resolution Chromatography: A Practical Approach (Practical Approach Series)
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Isolating and purifying proteins is a key step for a wide range biological experiments, and researchers require quick and reliable techniques. This practical volume fits this need. The first section, devoted to techniques and equipment, covers initial preparation, microscale techniques, detector technologies, and capillary electrophoresis and its associated techniques. The second section then covers a range of chromatographic procedures based on the interaction of a specific ligand with its target protein or other macromolecule. Some chapters cover non-specific interactions using peptides, inhibitors, and antibodies as the affinity ligand, while others focus on specific groups of molecules, including oligosaccharides and glycosylated proteins, nucleotide-binding proteins, proteins binding free and chelated metal ions, and DNA-binding proteins.

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Paul Millner is at University of Leeds.

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"Laboratory workers will find this a useful guide to the development of effective purification protocols" Aslib Book Guide"

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9780199636488: High Resolution Chromatography: A Practical Approach (Practical Approach Series)

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ISBN 10:  0199636486 ISBN 13:  9780199636488
Publisher: Oxford University Press, 1999
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Book Description Oxford University Press, UK, 1999. Hardcover. Condition: Near Fine. 0199636494. Pictorial board hardcover, from closed pharmaceutical company library. 311 pages, index, refs, list of Suppliers, illustrated with charts, tables and diagrams. Provides information for most experimental situations to enable rapid and effective purification. The first four chapters deal with the instrumental aspects of high resolution chromatography starting with the initial clean up steps prior to separation in chapter 1. Chapter 2 deals with microscale techniques, then chapter 3 describes the detector technologies that can determine information about the separated molecules. The final chapter in this section cover capillary electrophoresis and its associated techniques. The remaining chapters cover a range of chromatographic procedures based on the interaction of a specific ligand with its target protein or other macromolecule. Some chapters cover non-specific interactions using peptides, inhibitors, and antibodies as the affinity ligand while others focus on specific groups of molecules: oligosaccharides and glycosylated proteins, nucleotide-binding proteins, proteins binding free and chelated metal ions, and DNA binding proteins. Contents as new, clean, tight and bright. Book. Seller Inventory # 17460

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