Membrane Transport is targeted towards researchers with an interest in the mechanism of solute transport across biological membranes. Its scope is broad, ranging from the techniques required to study transport itself, through the expression, purification and reconstitution of transporters, to techniques for investigation of their structures. As such, it not only proves the necessary technical grounding for newcomers to the field, but should also be of value to "old-hands" wishing to get up to date with recent developments in these areas. While some of the approaches described require sophisticated equipment (e.g. a stopped-flow fluorimeter), most of the protocols can be implemented in any well- found laboratory. Preparation of this volume comes at a time when a result of genome sequencing our knowledge of membrane transporter sequences is far outstripping our understanding of their molecular mechanisms. Our hope is that this book will help future researchers to redress this imbalance.
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Stephen A. Baldwin, School of Biochemistry and Molecular Biology, University of Leeds.
"As expected from this long-running series, the high standard of detailed, easy-to-access protocols has been maintained...a useful guide and stimulant to those laboratories now prepared to get stuck into the analysis of membrane proteins at the structural level."Trends in Biochemical
Sciences
"The timing is right for a Practical Approach entry in this field ... The guide is well-organised and easy to use ... Reseachers who are currently working in the area of membrane transport (or just thinking about it) will want to obtain a copy of Membrane Transport:A Practical Approach for
their reference libraries."Biochemistry and Molecular Biology Education
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