Immunoassays: A Practical Approach (The ^APractical Approach Series) - Hardcover

Hardcover

ISBN 10: 0199637113 ISBN 13: 9780199637119

Publisher: Oxford University Press, 2000

Synopsis

Immunoassays is the first practical volume designed to help any biologist develop an immunoassay of any common format for any suitable analyte. The basic principles are described and the choices of assay types and formats listed. Methods for raising and for characterizing antibodies, for preparing radioactive, enzymatic, fluorescent and other labels, are described. Solid-phase reagents, standards and immunogens are explained, with procedures for their preparation. But most components can be obtained commercially and comprehensive lists of suppliers are given. Approaches to combining the reagents and optimizing the required assay are described, including how to eliminate common interferences, and thorough validation procedures are given. Manual and computer methods for the calculation of concentrations are explained. Since only assays that are within control give valid results, a graded and comprehensive approach to internal and external quality assurance methods is detailed, including how to prepare control samples.

About this title

About the Author

Jim Gosling, Department of Biochemistry, National University of Ireland.

Edition details

• Publisher: Oxford University Press
• Publication date: 2000
• Language: English
• ISBN 10: 0199637113
• ISBN 13: 9780199637119
• Binding: Hardcover
• Number of pages: 304
• Editor: Gosling James P.