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This convenient laboratory manual covers the theory and application of all the commonly used FISH procedures for both the research and clinical service laboratory. Readers are provided with the theoretical principles which underlie each procedure, and are then guided through the protocols in a systematic, easy to follow manner. Because FISH procedures are particularly sensitive to background and subtle variations in sample quality, the trouble-shooting sections are invaluable to the beginner interested in developing FISH capabilities in their laboratory. Areas covered include probe production and labeling; FISH gene mapping; murine, fiber, and RNA FISH; CGH and microchips; 3D FISH; SKY and M-FISH; FISH and microarrays; and FISH in clinical cytogenetics.
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Barbara G Beatty is at Health Sciences Research Facility, University of Vermont, USA. Sabine Mai is at Manitoba Institute of Cell Biology, Winnipeg, Canada.Review:
"The book will be of value to researchers and students in molecular pathology, cancer biology, and genomics."--SciTech Book News
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