Protocols and Applications in Enzymology - Softcover

Belorkar, Seema Anil; Jogaiah PhD, Sudisha

Softcover

ISBN 10: 0323912680 ISBN 13: 9780323912686

Publisher: Academic Press, 2021

Synopsis

Protocols and Applications in Enzymology provides instruction on the experimental procedures of enzyme isolation techniques, innovative screening techniques, and instrument enabled enzyme assays and their underlying principles, among other protocols. The book serves as a one-stop solution for those working with different enzyme protocols in the fields of biochemistry, microbiology, biotechnology and allied subjects. Each chapter offers a full overview of protocol key resources, materials required, quantifiable and statistical analysis, optimization and troubleshooting, safety considerations, and standards. Applications are discussed across distribution and diversity of microbial enzymes, enzyme screening, enzymes in solid state fermentations, enzyme assays, enzyme kinetics, and biotechnological uses.

• Provides step-by-step instruction on enzyme protocols and applications, with actionable discussions of needed resources, materials, quantification and statistical analysis, optimization and troubleshooting, safety considerations and standards
• Presents easy to read, reproducible protocols for researchers and students across academia and industry
• Includes color diagrams that illustrate key concepts

About this title

About the Authors

Dr. Seema Anil Belorkar is Assistant Professor at the PG Department of Microbiology and Bioinformatics, Atal Bihari Vajpayee University, Bilaspur, with expertise in bioactive molecule production and enzymology. She has published 20 publications in various national and international journals, and has successfully completed 4 research projects with involvin production of enzymes and their application in agro-industrial wastes. Dr. Belorkar has contributed to 4 chapters in various books published by Springer, Wiley-Blackwell, and Elsevier. She has received 3 awards in the field of teaching and research, and has been a reviewer for articles in BMC Plant Biology, the Indian journal of Pharmaceutical Sciences and other journals of repute.

Dr. Sudisha Jogaiah is a Fellow of the National Academy of Sciences (FNASc), the Agricultural Sciences (FNAAS), and the National Academy of Biological Sciences (FNABS). He is also a Bridge Fellow, Government of Japan, and serves as a Visiting Professor at Yamaguchi University, Japan, and Nanjing Agricultural University, China. In 2022, he joined the Department of Environmental Science at the Central University of Kerala as an Associate Professor. He has been listed among the World's Top 2% of Scientists by Stanford University for 3 consecutive years. His research interests include plant pathology, plant–microbe interactions, plant defense signaling, biocontrol agents, biogenic nanoparticles for induced resistance in plants, and bioremediation. The technological quality and scientific rigor of his laboratory are evident from his extensive publication record and the prestigious national and international awards received.

From the Back Cover

Protocols and Applications in Enzymology describes and provides instruction in the experimental procedures of enzyme isolation techniques, innovative screening techniques, and instrument enabled enzyme assays and their underlying principles, among other protocols. It serves as a one-stop solution for those working with different enzyme protocols in the fields of biochemistry, microbiology, biotechnology, and allied subjects. Each chapter offers a full overview of protocol key resources, materials required, quantifiable and statistical analysis, optimization and troubleshooting, safety considerations, and standards. Applications are discussed across distribution and diversity of microbial enzymes, enzyme screening, enzymes in solid state fermentations, enzyme assays, enzyme kinetics, and a range of other biotechnological uses.

Edition details

• Publisher: Academic Press
• Publication date: 2021
• Language: English
• ISBN 10: 0323912680
• ISBN 13: 9780323912686
• Binding: Paperback
• Edition number: 1
• Number of pages: 266