Basic Methods in Molecular Biology explores the core of biology's latest revolution: genetic technology. This field's advancements have transformed not just biologists' work, but also their thought processes. Never before have scientists from diverse disciplines converged on a single, specialized field to learn and adopt its technologies. This comprehensive book consists of 21 chapters, starting with three introductory ones that cover molecular biology fundamentals, essential tools, and general usage guidelines. Subsequent chapters delve into cloning vectors and bacterial cells, DNA preparation from eukaryotic cells, nucleic acid probing, plasmid DNA preparation, DNA restriction fragment preparation, DNA purification, and RNA preparation and analysis from eukaryotic cells. Other topics include DNA preparation from bacteriophage clones, cloning DNA from the eukaryotic genome, subcloning into plasmids, M13 cloning and sequencing, cloned DNA further characterization, mammalian cell transfection, protein methods, general methods, and specialized methods. This book is tailored for professionals in biology and molecular genetics.
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The new edition of this popular book emphasizes the decisions that need to be made to select one procedure over another in the everyday workings of the molecular biology laboratory. Trouble-shooting problems that may emerge when performing such methods are included. New section on the preparation and analysis of differential cDNA libraries.
Leonard G. Davis, PhD (David Mahoney Institute of Neurological Sciences, University of Pennsylvania, Philadelphia) Michael Kuehl, MD (Laboratory of Biological Chemistry, National Cancer Institute, Bethesda, Maryland) James F. Battey, MD, PhD (Laboratory of Biological Chemistry, National Cancer Institute, Bethesda, Maryland)
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