Assay Development: Fundamentals and Practices

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9780470191156: Assay Development: Fundamentals and Practices
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Essential principles and practice of assay development

The first comprehensive, integrated treatment of the subject,Assay Development: Fundamentals and Practices covers the essentialsand techniques involved in carrying out an assay project in eithera biotechnology/drug discovery setting or a platform setting.

Rather than attempting comprehensive coverage of all assaydevelopment technologies, the book introduces the most widely usedassay development technologies and illustrates the art of assaydevelopment through a few commonly encountered biological targetsin assay development (e.g., proteases, kinases, ion channels, and Gprotein-coupled receptors). Just enough biological background forthese biological targets is provided so that the reader can followthe logics of assay development. Chapters discuss:

  • The basics of assay development, including foundational conceptsand applications

  • Commonly used instrumental methods for both biochemical assaysand cell-based assays

  • Assay strategies for protein binding and enzymatic activity

  • Cell-based assays

  • High-throughput screening

An in-depth study of the now popular Caliper's off-chip kinaseassay provides an instructive, real-world example of the assaydevelopment process.

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About the Author:

Ge Wu, PhD, is Director of Assay Development at IrisInternational. He obtained his doctorate in biochemistry from UCLA,performed postdoctoral work at Harvard Medical School, and was amember of the faculty at Cornell University Medical College. Priorto joining Iris International, he worked at Merck & Co.,Caliper Technologies, Amphora Discovery, and FivePrimeTherapeutics. He is the founding Director of Assay Development andScreening at FivePrime Therapeutics. Dr. Wu is a leading figure inassay development and screening in biopharmaceutical discoveryusing primary cell assay and the author of an acclaimed review inthis subject.

Review:

"Although it is not an easy task to satisfy the demands of all scientists, the book Assay Development is an important treaise for scientits of modern biology". (ChemBioChem, 7 March 2011)

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Book Description John Wiley and Sons Ltd, United States, 2010. Hardback. Condition: New. 1. Auflage. Language: English. Brand new Book. The first comprehensive, integrated treatment of the subject, Assay Development: Fundamentals and Practices covers the essentials and techniques involved in carrying out an assay project in either a biotechnology/drug discovery setting or a platform setting. After discussing the basics, it explains the treatment of assays for purified protein, cell-based assays, and high-throughput screening. It includes good practices and case studies. This is a premier resource for biotechnologists, analytical chemists, biochemists, medicinal chemists, toxicologists, and others, and an excellent text for professors preparing students to enter the biotechnology and pharmaceutical industries. Seller Inventory # AAH9780470191156

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Book Description John Wiley and Sons Ltd, United States, 2010. Hardback. Condition: New. 1. Auflage. Language: English. Brand new Book. The first comprehensive, integrated treatment of the subject, Assay Development: Fundamentals and Practices covers the essentials and techniques involved in carrying out an assay project in either a biotechnology/drug discovery setting or a platform setting. After discussing the basics, it explains the treatment of assays for purified protein, cell-based assays, and high-throughput screening. It includes good practices and case studies. This is a premier resource for biotechnologists, analytical chemists, biochemists, medicinal chemists, toxicologists, and others, and an excellent text for professors preparing students to enter the biotechnology and pharmaceutical industries. Seller Inventory # AAH9780470191156

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Book Description John Wiley and Sons Ltd, United States, 2010. Hardback. Condition: New. 1. Auflage. Language: English. Brand new Book. The first comprehensive, integrated treatment of the subject, Assay Development: Fundamentals and Practices covers the essentials and techniques involved in carrying out an assay project in either a biotechnology/drug discovery setting or a platform setting. After discussing the basics, it explains the treatment of assays for purified protein, cell-based assays, and high-throughput screening. It includes good practices and case studies. This is a premier resource for biotechnologists, analytical chemists, biochemists, medicinal chemists, toxicologists, and others, and an excellent text for professors preparing students to enter the biotechnology and pharmaceutical industries. Seller Inventory # BTE9780470191156

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