Assay Development: Fundamentals and Practices

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9780470191156: Assay Development: Fundamentals and Practices

Essential principles and practice of assay development

The first comprehensive, integrated treatment of the subject, Assay Development: Fundamentals and Practices covers the essentials and techniques involved in carrying out an assay project in either a biotechnology/drug discovery setting or a platform setting.

Rather than attempting comprehensive coverage of all assay development technologies, the book introduces the most widely used assay development technologies and illustrates the art of assay development through a few commonly encountered biological targets in assay development (e.g., proteases, kinases, ion channels, and G protein-coupled receptors). Just enough biological background for these biological targets is provided so that the reader can follow the logics of assay development. Chapters discuss:

  • The basics of assay development, including foundational concepts and applications

  • Commonly used instrumental methods for both biochemical assays and cell-based assays

  • Assay strategies for protein binding and enzymatic activity

  • Cell-based assays

  • High-throughput screening

An in-depth study of the now popular Caliper's off-chip kinase assay provides an instructive, real-world example of the assay development process.

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About the Author:

Ge Wu, PhD, is Director of Assay Development at Iris International. He obtained his doctorate in biochemistry from UCLA, performed postdoctoral work at Harvard Medical School, and was a member of the faculty at Cornell University Medical College. Prior to joining Iris International, he worked at Merck & Co., Caliper Technologies, Amphora Discovery, and FivePrime Therapeutics. He is the founding Director of Assay Development and Screening at FivePrime Therapeutics. Dr. Wu is a leading figure in assay development and screening in biopharmaceutical discovery using primary cell assay and the author of an acclaimed review in this subject.

Review:

"Although it is not an easy task to satisfy the demands of all scientists, the book Assay Development is an important treaise for scientits of modern biology". (ChemBioChem, 7 March 2011)

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