This important contribution to the scientific community explains various aspects of reverse-phase separations. How to Use Reverse-Phase HPLC surveys the basics of liquid chromatography and summarizes the theoretical aspects of reverse-phase HPLC. Chapters also discuss: the influence of stationary and mobile phases on the efficiency and selectivity of the separations; the use of conventionally used and special reverse phase packings as well as that of masking agents added in the mobile phase; the evaluation of column performance in reverse phase chromatography; the applicability of special methods and techniques in RP-HPLC; the most important practical aspects of phase system optimization; and HPLC method validation summarizing the practical approaches recommended for the design and performance of validation experiments.
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