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Over the last decade, advances in science and technology have profoundly changed the face of light microscopy. Research scientists need to learn new skills in order to use a modern research microscope–skills such as how to align microscope optics and perform image processing. Fundamentals of Light Microscopy and Electronic Imaging explores the basics of microscope design and use. The comprehensive material discusses the optical principles involved in diffraction and image formation in the light microscope, the basic modes of light microscopy, the components of modern electronic imaging systems, and the image processing operations necessary to acquire and prepare an image.
Written in a practical, accessible style, Fundamentals of Light Microscopy and Electronic Imaging reviews such topics as:
Each chapter includes practical demonstrations and exercises along with a discussion of the relevant material. In addition, a thorough glossary assists with complex terminology and an appendix contains lists of materials, procedures for specimen preparation, and answers to questions.
An essential resource for both, experienced and novice microscopists.
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"This well written text provides a clear, uncluttered overview of the principles and practice of modern light microscopy. It contains many helpful teaching exercises and diagrams. It should prove useful in a wide range of courses from the undergraduate to the postgraduate level." (Kenneth R. Spring, Ph.D., Author)
"Douglas Murphy's book, Fundamentals of Light Microscopy and Electronic Imaging, is a welcome tool to introduce students in the biological sciences to the fundamentals of modern light microscopy and its augmentation by electronic imaging methods"..."this book provides a comprehensive introduction to the range of light-based methods available today"..."Each section has illustrative exercises and/or demonstrations, making this book readily adaptable to course work. " (Gordon W. Ellis, University of Pennsylvania)
"Given the remarkable resurgence of interest in biological microscopy during the past few years and the genuine need for a text in this field, Murphy's book will be warmly welcomed by all cell biologists who use microscopes in their research." (Joseph G. Gall, American Cancer Society Professor of Developmental Genetics, Carnegie Institution)From the Author:
The book retains a tested tradition of explaining image formation in the light microscope. Essential information is included about: aligning the microscope for different modes of light microscopy; the selection of filters, illuminators and fluorescent dyes; preparation and protection of cells for microscope observation, and other topics. Instructions are given on how to evaluate the qualities of a CCD camera and an electronic image, and how to operate and use electronic imaging systems.
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