Culture of Epithelial Cells - Softcover

9780471401216: Culture of Epithelial Cells

Softcover

ISBN 10: 0471401218 ISBN 13: 9780471401216

Publisher: Wiley-Liss, 2002

"...a wonderful compendium of current in vitro approaches that will be a useful resource to those just starting to work with an epithelial cell system as well as those that have been working with them for years and years."
--Pharmaceutical Research

This completely revised and expanded new edition provides detailed descriptions of fundamental and practical aspects relating to the in vitro cultivation of disparate types of epithelia. In recent years, the use of epithelial cell culture in cell biology and tissue engineering has increased dramatically. This revision reflects those advances by including new chapters on the culture of animal and human hepatocytes, kidney epithelium, and bladder epithelium. Each chapter provides an introductory review of the principles and advantages of the particular method, followed by detailed protocols, practical tips, alternate methods, and a useful list of materials and suppliers.

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From the Author:

A detailed introduction to the biology and methodology of epithelial cell culture, with most of the main epithelial cell types covered in separate chapters, each with reagents, media and step-wise protocols, and a supplementary chapter reviewing methods and presenting some protocols for the culture of less frequently used epithelia.

From the Back Cover:

"It is now the age of the specialized cell in culture," R. Ian Freshney wrote in the preface to the first edition of Culture of Epithelial Cells. Realizing the inadequacy of studying all types of cells at random in a culture, it became imperative for researchers to learn how unique cells perform under a wide variety of conditions. Now the Second Edition of Culture of Epithelial Cells--devoted to those cells that line the body, internally and externally, protecting surfaces and secreting or absorbing material--advances this ethic, providing completely revised, detailed descriptions of fundamental and practical aspects relating to the in vitro cultivation of disparate types of epithelia.

Each chapter of the Second Edition is devoted to a different type of epithelium--epidermal, mammary, cervical, prostatic, oral, intestinal, pulmonary, hepatic, and urothelial. R. Ian Freshney also contributes a final chapter on a number of epithelia not covered in the main chapters, presenting them together in review form with additional protocols. Other features of the Second Edition of Culture of Epithelial Cells include:

Proven methods refined by the authors, all well-established researchers
Alternate techniques coupled with critical comparative analyses
Logical anatomical organization by tissue type
Stepwise culture protocols conveniently highlighted
New chapters on the culture of oral, alveolar, intestinal, and urothelial epithelium
In-depth analysis of cell-cell interaction in skin

Displaying the clear, easy-to-follow style that has become a trademark of the Culture of Specialized Cells series, the Second Edition of Culture of Epithelial Cells remains an essential resource for researchers in cell and developmental biology, cancer biology, tissue engineering, biochemistry, and genetics, where the work requires the cultivation or manipulation of cells from the epithelium.

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