Items related to Protein Methods
Here is a step-by-step guide to the general methods used for protein research. For each method, all reagents and items of equipment are described; a detailed protocol is presented; and potential problems, and pitfalls are discussed. Basic methods for extracting, handling, storing and measuring the concentration of proteins are presented. Various approaches to the study of proteins, using gel electrophoresis and related methods, are also covered. It will allow a novice to perform the various procedures. Experienced research workers will find critical information concerning advanced procedures assembled in a single source for the first time.
"synopsis" may belong to another edition of this title.
From the Back Cover:
Protein Methods by Daniel M. Bollag, Michael D. Rozycki, Stuart J. Edelstein
Reviews from the first edition
"...it is very well written...would certainly be of use to undergraduate and postgraduate students entering the complex world of protein preparation and to more experienced scientists entering this field for the first time." --Biotechnology and Applied Biochemistry
"The book presents these topics with loads of practical detail, so that the reader has little need to consult other reference sources to carry out the techniques described. All in all, a useful book..." --Theoretical & Applied Genetics
"...clearly written with protocols that are easy to follow and the text is well spread-out and easy to read...all methods are fully referenced...a useful book for beginners at a reasonable price." --FEBS Letters
This revised and expanded Second Edition of Protein Methods remains the first source for a complete summary of tested and proven protein techniques. Now divided into two parts, the book begins with the essential chapters from the first edition, updated to reflect important changes in methodology. The chapter on protein isolation includes a new section focusing on the isolation of proteins from inclusion bodies. In the second section, four new chapters are devoted to protein purification and crystallization.
Chapters include
* Preparation for Protein Isolation
* Protein Extraction and Solubilization
* Protein Concentration Determination
* Concentrating Protein Solutions
* Gel Electrophoresis Under Denaturing Conditions
* Gel Electrophoresis Under
...and feature
* reagent items and equipment required for each method
* chapter "view-at-a-glance" tabs
* illustrations for all gel applications and other methods
* complete supplier list
* appendices of basic chemical parameters and molecular data
* key references
* descriptive tables
* Safety notes.
Reviews from the first edition
"...it is very well written...would certainly be of use to undergraduate and postgraduate students entering the complex world of protein preparation and to more experienced scientists entering this field for the first time." --Biotechnology and Applied Biochemistry
"The book presents these topics with loads of practical detail, so that the reader has little need to consult other reference sources to carry out the techniques described. All in all, a useful book..." --Theoretical & Applied Genetics
"...clearly written with protocols that are easy to follow and the text is well spread-out and easy to read...all methods are fully referenced...a useful book for beginners at a reasonable price." --FEBS Letters
This revised and expanded Second Edition of Protein Methods remains the first source for a complete summary of tested and proven protein techniques. Now divided into two parts, the book begins with the essential chapters from the first edition, updated to reflect important changes in methodology. The chapter on protein isolation includes a new section focusing on the isolation of proteins from inclusion bodies. In the second section, four new chapters are devoted to protein purification and crystallization.
Chapters include
* Preparation for Protein Isolation
* Protein Extraction and Solubilization
* Protein Concentration Determination
* Concentrating Protein Solutions
* Gel Electrophoresis Under Denaturing Conditions
* Gel Electrophoresis Under
...and feature
* reagent items and equipment required for each method
* chapter "view-at-a-glance" tabs
* illustrations for all gel applications and other methods
* complete supplier list
* appendices of basic chemical parameters and molecular data
* key references
* descriptive tables
* Safety notes.
"About this title" may belong to another edition of this title.
- PublisherWiley
- Publication date1990
- ISBN 10 0471568716
- ISBN 13 9780471568711
- BindingPaperback
- Number of pages242
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Protein Methods
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ISBN 10: 0471568716
ISBN 13: 9780471568711
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Protein Methods
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(1990)
ISBN 10: 0471568716
ISBN 13: 9780471568711
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Book Description Plastic comb binding. Condition: Very Good+. Dust Jacket Condition: No Dust Jacket. Rubbing and toning overall and a blind ex-libris stamp on the half-title. Interior pages clean and unmarked. Photos on request. International shipping billed at cost. ; 4to 11" - 13" tall; 242 pages. Seller Inventory # 87664
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