Protein Purification: Principles, High Resolution Methods, and Applications (Methods of Biochemical Analysis) - Hardcover

Hardcover

ISBN 10: 0471746614 ISBN 13: 9780471746614

Publisher: Wiley, 2011

Synopsis

The authoritative guide on protein purification―now completely updated and revised

Since the Second Edition of Protein Purification was published in 1998, the sequencing of the human genome and other developments in bioscience have dramatically changed the landscape of protein research. This new edition addresses these developments, featuring a wealth of new topics and several chapters rewritten from scratch.

Leading experts in the field cover all major biochemical separation methods for proteins in use today, providing professionals in biochemistry, organic chemistry, and analytical chemistry with quick access to the latest techniques. Entirely new or thoroughly revised content includes:

• High-resolution reversed-phase liquid chromatography

• Electrophoresis in gels

• Conventional isoelectric focusing in gel slabs and capillaries and immobilized pH gradients

• Affinity ligands from chemical and biological combinatorial libraries

• Membrane separations

• Refolding of inclusion body proteins from E. coli

• Purification of PEGylated proteins

• High throughput screening techniques in protein purification

• The history of protein chromatography

About this title

About the Author

JAN-CHRISTER JANSON is Professor Emeritus at the Department of Physical and Analytical Chemistry, Uppsala University, Uppsala, Sweden.

From the Back Cover

The authoritative guide on protein purification now completely updated and revised

Since the Second Edition of Protein Purification was published in 1998, the sequencing of the human genome and other developments in bioscience have dramatically changed the landscape of protein research. This new edition addresses these developments, featuring a wealth of new topics and several chapters rewritten from scratch.

Leading experts in the field cover all major biochemical separation methods for proteins in use today, providing professionals in biochemistry, organic chemistry, and analytical chemistry with quick access to the latest techniques. Entirely new or thoroughly revised content includes:

• High-resolution reversed-phase liquid chromatography

• Electrophoresis in gels

• Conventional isoelectric focusing in gel slabs and capillaries and immobilized pH gradients

• Affinity ligands from chemical and biological combinatorial libraries

• Membrane separations

• Refolding of inclusion body proteins from E. coli

• Purification of PEGylated proteins

• High throughput screening techniques in protein purification

• The history of protein chromatography

From the Inside Flap

The authoritative guide on protein purification—now completely updated and revised

Since the Second Edition of Protein Purification was published in 1998, the sequencing of the human genome and other developments in bioscience have dramatically changed the landscape of protein research. This new edition addresses these developments, featuring a wealth of new topics and several chapters rewritten from scratch.

Leading experts in the field cover all major biochemical separation methods for proteins in use today, providing professionals in biochemistry, organic chemistry, and analytical chemistry with quick access to the latest techniques. Entirely new or thoroughly revised content includes:

• High-resolution reversed-phase liquid chromatography

• Electrophoresis in gels

• Conventional isoelectric focusing in gel slabs and capillaries and immobilized pH gradients

• Affinity ligands from chemical and biological combinatorial libraries

• Membrane separations

• Refolding of inclusion body proteins from E. coli

• Purification of PEGylated proteins

• High throughput screening techniques in protein purification

• The history of protein chromatography

Edition details

• Publisher: Wiley
• Publication date: 2011
• Language: English
• ISBN 10: 0471746614
• ISBN 13: 9780471746614
• Binding: Hardcover
• Edition number: 3
• Number of pages: 544
• Editor: Janson Jan-Christer