HPLC: A Practical User's Guide

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9780471754015: HPLC: A Practical User's Guide
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This Second Edition of the classic handbook details how to set upan HPLC system that capitalizes on the latest innovations. Itcovers new techniques in high-temperature, micro-flow, andultra-fast chromatography, the linking of an HPLC to a massspectrometer, and more. Complete with a CD-ROM and appendices, thisguide has everything chromatographers need to know to confidentlyseparate, identify, purify, and quantify compounds.

Note: CD-ROM/DVD and other supplementary materials arenot included as part of eBook file.

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From the Back Cover:

Everything you need to set up an HPLC systemthat takes advantageof the latest innovations

Here is the perfect handbook for both novice and experiencedchromatographers. For novices, HPLC, Second Edition cuts throughthe technical detail, theory, and confusing variety of columntypes, enabling them to easily set up and maintain an HPLC system.For more experienced practitioners, the book explains how to fullyexploit the most recent innovations and improvements in columntechnology. The latest information and techniques inhigh-temperature, micro-flow, and ultra-fast chromatography, aswell as the linking of an HPLC to a mass spectrometer, representjust some of the new material featured in this Second Edition.

Readers benefit from the author's twenty-five years ofexperience in HPLC, first as a user, then as a field sales andapplication support representative, and finally as a teacher andconsultant. The book's wealth of tips and troubleshooting adviceoriginate from the author's own laboratory and field work.

HPLC's structure is carefully designed to give readers asolid foundationin the basics and then methodically build andrefine their skills:

  • Part I, HPLC Primer, presents the essentials needed toget an HPLC system up and running
  • Part II, HPLC Optimization, explains how to make thebest use of the common columns and troubleshoot problems
  • Part III, HPLC Utilization, focuses on real-worldapplications, including interfacing HPLC systems with computers androbotic workstations

A CD-ROM with the author's PowerPoint® presentation,Practical Course in HPLC, is included with the book. Plus, newappendices offer answers to frequently asked questions, tables ofsolvents and volatile buffers, an updated glossary of terms, andlaboratory experiments. In short, everything that you need to knowto confidently separate, identify, purify, and quantify compoundsis here.

About the Author:

MARVIN C. McMASTER, PhD, is a consultant as well as an Adjunct Professor of Chemistry at the University of Missouri-St. Louis. Dr. McMaster has served as a researcher and product developer for DuPont, Kraft Foods, and Ciba-Geigy. Among his other publications, Dr. McMaster is author of LC/MS: A Practical User's Guide and coauthor of GC/MS: A Practical User's Guide (both published by Wiley).

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