Gene isolation has now become an essential step in dissecting the molecular control of fundamental plant processes enabling their manipulation and improvement by genetic engineering. However, the isolation of the "gene of interest" often represents a formidable task, similar to looking for a "needle in a haystack." Many approaches are well established, but new approaches and techniques have been developed in recent years which can streamline the progress of a gene cloning project. Plant Gene Isolation: Principles and Practice, written by a team of international researchers, provides a comprehensive guide to the history, theoretical background and application of experimental methods needed to undertake the isolation of genes from plants. Laboratory procedures are described, including the advantages and disadvantages of methods and advice on which technique to use. Information on useful sources and suppliers is also given.
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